Primary Study On Gene Cloning And Expression Of Neisseria Gonorrhoea Surface Protein A And Deinococcus Radiodurans Superoxide Dismutase In Lactococcus Lactis

ObjectiveTo construct the expression recombinants by cloning the genes of Neisseria gonorrhoea surface protein A (NspA) and Deinococcus radiodurans Mn superoxide dismutase (Mn-SOD) into the cytoplasmic vector pMG36e and secretion vector pVE5523 of lactic acid bacteria(LAB). The recombinants were transformed into Lactococcus lactis by electroporation and the expressed fusion NspA and SOD were studied primarily. This research will play a basis for screening the expression vector for two genes, provide a reference for developing the bi-functional LAB live vaccine of Neisseria gonorrhoea outer membrane protein, and lay a groundwork for the further study on the influence of the expressed fusion SOD on LAB and for the development of food grade SOD product or microecology formulation.Methods1. Construction and expression of the recombinants pMG36e-nspA and pMG36e-sod in L. lactis1) Optimize the condition of extracting the genome DNA of N. gonorrhoea and D. radiodurans by CTAB method. Design the...