| Cell immunity function mediated by T lymphocyte and cytotoxicity of NK cell could be inhibited in patients with advanced digestive tract cancer, because tumor cells could generate and synthesize soluble immunosuppressor, which induce suppressor T cells (CD8~+) and inhibit maturation of helper T cells (CD4~+). This is escape immunosurveillance of tumor.Chemotherapy plays an important role in the treatment of digestive tract cancer. However, the bottleneck of chemotherapy is that not only malignant but many normal cells including immune cells are not spared. In addition to the negative effect of tumor cells, patients had a relatively low immune function. After chemotherapy, this immune depression state was strengthened. However, different viewpoints were proposed from latest research. It is indicated that in the course of intravenous chemotherapy, patients of effective or non-effective groups had definitely different immunological state.Our research is designed to use future cohort studies, by monitoring the change pattern of the phenotype of T cells in peripheral blood and cytokine in plasma in patients with digestive tract cancer, in order to discuss the different immunological state in patients before and after chemotherapy and its clinical significance.(1) According to multiple color immunofluorescent method and FCM analysis, the percentages of CD3~+, CD4~+, CD4~+CD28~+ and CD8~+CD28~+ Cell and ratio of CD4/CD8 were decreased with statistical significance in patients with advanced digestive tract cancer compared with normal person. All the changes were related with clinical stage of tumor. After intravenous chemotherapy, in early stage (about 1 -2 weeks) patients were in an immunosuppression state, while in 3 weeks later patients with effective results had recovery of immunity, such as proportion of CD3~+,CD4~+, CD4~+CD28~+ and CD8~+CD28~+ Cell and ratio of CD4/CD8 was significantly increased compared with patients before chemotherapy and was close to normal control groups. On the contrary, the expression of membrane molecular in non-effective patients was decreased all the way. All these data... |
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