Pharmaceutical Research On The Added Drugs In JiaWei PiPa QingFei Capsule And Analysis Of Flavor Components Of Fenugreek (Trigonella Foenum-graecum L.)

The pharmaceutical research on the added drugs (Radix Scutellaria,Fructus Gardeniae,Fructus Crataegi and Gypsum Fibrosum) of the JiaWeiPiPaQingFei capsule is carried out in this thesis. Acne vulgaris is a kind of chronic sebaceous gland folliculitis on the skins, with high incidence. Owing to the complicated pathogeny of acne, the Western medicine is used generally to cure the acne with some side effects and limitations. Traditional Chinese Medicine has been used to cure acne for centuries-old history, but many of them are merely prescriptions in clinic. Hasn't any kind of production of Traditional Chinese Medicine been prepared to cure acne at present. PiPaQingFei decoction is used to cure the acne in clinic effectivelly .But it has not been developed as Traditional Chinese Medicine's new preparations which is easy to be used and produced.On the basis of classic prescription PiPaQingFei decoction,the research group has prepared the JiaWeiPiPaQingFei capsule to cure acne vulgaris,adding some kinds of crude drugs with modern clinical experience. The pharmaceutical study on the added drugs is the main content in this thesis.Analysis study of the principal flavor components of fenugreek is also carried out in this thesis. Fenugreek (Trigonella foenum-graecum L.) is originally cultivated in Mediterranean countries and used as spice and crude drugs since ancient times. With enjoyable burnt sugar type sweet-smelling, haut bitter taste, fenugreek oil is widely used in food and tobacco industry. There is no report on the research of principal flavor components of fenugreek at home.The main contents and conclusions are as follows:1 .According to physico-chemical property of active components of each crude drugs , The process of extracting active components in group is decided :Radix Scutellaria is extracted alone , Fructus Gardeniae and Fructus Crataegi is extracted together, Gypsum Fibrosum is used as adjuvant.2. Using the yield of baicalin as technology target,The optimal process to extract and purify Radix Scutellaria is the crude drugs being extracted twice with one hour each, after adding 10 times water first, and 8 times water second. The extraction is filtered and centrifugated .The supernate is preservation at the temperature 60℃about 30 minutes after the pH is adjust to 1.5 with hydrochloric acid. To be centrifugated again, the sediment is dissolved after adding 10 times water, and the pH of solution arrives to 7.0. The solution is centrifugated after adding the same volume ethanol. The supemate is preservation at the temperature 60℃about 30 minutes after the pH is adjust to 1.0 with hydrochloric acid and centrifugated. The extractum of Radix Scutellaria can be finally obtained after washing the sediment by water until neutrality and dried at the temperature 60℃. The yield of extractum of Radix Scutellaria is 0.14g/g crude drugs and the yield of baicalin is 119.57mg/g. The content of baicalin in extractum of Radix Scutellaria is 86.6%.3.Using the yield of gardenoside and total flavones as technology target, the optimal process to extract and purify Fructus Gardeniae and Fructus Crataegi is the crude drugs are extracted three times, 1.5 hours each, with 10 times 60% ethanol. The extracts is purified by macroporous adsorptive resins. By resin selected, dynamic absorption and orthogonal experiments, the optimal purified process is decided: every 60mL sample solution of Fructus Gardeniae and Fructus Crataegi (0.05g cude drug /ml,pH3～4) to deal with 5g AB-8, resin(humid weight). The flow rate is 0.5 ml/min. After adsorbing with the resin, eluting with 50% alcohol, drying at the temperature 60℃, the extractum of Fructus Gardeniae and Fructus Crataegi can be obtained. The yield of extractum of Fructus Gardeniae and Fructus Crataegi is 0.08g/g crude drugs. The yield of gardenoside is 21.8mg/g crude drugs and total fiavones is 22.Smg/g. The content of gardenoside in extractum of Fructus Gardeniae and Fructus Crataegi is 27.3% and total flavones is 28.1%o4. Fructus Gardeniae in the extractum of Fructus Gardeniae and Fructus Crataegi is identified by TLC. The content of geniposide in this extractum is determined by HPLC The quality standards of the extractum of Fructus Gardeniae and Fructus Crataegi. was established. Fingerprint of Radix Scutellaria, the extractum of Radix Scutellaria was studied by HPLC, the results indicated that there was great dependablity in the HPLC fingerprint Radix Scutellaria and the extractum of Radix Scutellaria.5.The study of preliminary pharmacodynamics of added medicines, e. g. the ear edama of mice test, shows intermediate extractum of added drugs display good anti-flammaory effect. The diminution of the area of the Syrian Hamster flank sebaceous glands reveals that the added drugs can effect the level of androgenic in sebaceous gland.6.The optimal conditions for extracting of fenugreek oil from material with supercritical CO₂ fluids: extraction temperature 40℃, extraction pressure 25MPa, separation temperature 60℃, separation pressure one atmosphere pressure. Adding 50%(V/W) absolute alcohol as modifier, the highest extraction ratio is 8.95mg/g dry fenugreek seeds.7. Many fatty acids in Fenugreek oil extracted is identified by analysis using GC-MS after methyl esterified, and the main components are 8, 11-Octadecadienoic acid, 9-Octadecenoic acid, Hexadecanoic acid. The principal flavor components of fenugreek is undetected8. According to a literature, a method for determination of sotolone in fenugreek. is established. Sotolone was extracted from fenugreek by solvent and concentrated on Oldershow column. The structure of sotolone was identificated using GC/MS and the quantitative analysis of the content of sotolone determined using according to the extra-calibration curve by GC. The content of sotolone in fenugreek is reported for the first time at home, about 4.5mg/kg.