Microsatellite DNA Analysis For Genetic Background Monitoring Of Inbred Yuyi Hairless Mice

Aim: Adopting high-inbreeding animal can not only decrease experimental animal amount and experimental repetition times, but also improve accuracy, comparability and repetition of experimental result. The remarkable characteristics of inbred mice are gene purity and gene stability. There exist probabilities of genetic variation or genetic contamination during breeding, breeding conservation and production. Therefore, a strict, long-term, time genetic monitoring for inbred mice is needed. Traditional methods, such as skin grafting, biochemical markers and other ways by inferring from epitype changes to gene changes, have obvious limits. Because of microsatellite DNA being high polymorphism, extensive distribution, large quantity, relative stability, feasibility of amplification, it can directly reflect gene change at DNA level and reflect genetic profile and variant situation of genome much more generally. Microsatellite DNA has many merits, including test sensitivity, particularity, simplicity and convenience, time-efficiency. Thus microsatellite DNA will be an ideal genetic monitoring marker for inbred animal. Yuyi hairless mice (for short: YYHL mice ) is a mutant strain found by the Lab Animal Center of Henan Province during Kunming mice reproduction in 1990. YYHL mice is a new strain of genetical mutant that is different from nude mice in skin and hair structures. Inbred YYHL mice is widely applied to histology, tumour etiology, tumour therapy and other...