The Significance Of DNA Repair Protein In Gastric Carcinoma

Background and Objective: Gastric carcinoma is the most common malignant tumor, whose development maybe associated with the absence of DNA repair function of cells. There are lots of DNA repair forms. Mismatch repair (MMR) system could repair base mismatch when DNA is replicating and ensure integrality of DNA. Damage reverse (DR) could repair DNA damage caused by alkylating agent and oxidizing agent and so on which is the simplest form to sustain the normal framework of genes. Homologous recombinational repair (HR) could repair DNA double-strand breaks caused by DNA metabolism, ionization radiation and active oxygen, to sustain the integrality of genome and the normal expression of genes. Any abnormal expressions of DNA repair gene and their products may lead to the absence of DNA repair functions, accumulating of incorrect information and malignancy of cells. Absence of mismatch repair, damage reverse and homologous recombinational genes may lead to the occurrence of gastric carcinoma. This study is designed to answer the questions by detecting the protein expressions of MMR (hMSH2), DR (MGMT) and HR (XRCC3, XRCC3) protein in gastric carcinoma and the epithelia surrounding carcinoma and non carcinoma gastric mucosa (NCGM), and then to analyses the mechanism of gastric carcinoma.Materials and methods: The gastroscope biopsy speciments of Dalian city were collected from 56 gastric carcinomas and 30 gastritis mucosa. The expressions of hMSH2, MGMT, XRCC2 and XRCC3 protein were detected by immunohistochemistry (SP) method. Chi-square and Spearman is used for statistic analysis.Results: The positive expression rate of hMSH2 in carcinoma being...