The Preliminary Study For The Mechanism And Significances Of Hepatic Hydatid Cyst Calcification

AIM: To investigate the mechanism and significances of Hepatic Hydatid Cyst calcification.METHODS: 60 surgically excised pericysts were studied. To observe the distribution of calcium deposits in the pericystic layer of hepatic hydatid cysts, Von Kossa and Masson's Trichrome Stain were used ; Also the calcium metabolism related factors of the patient serium and the observation of computerized tomography ( CT) were retrospectively analysised;To detect the expression of osteopontin, proteins extracted from hydatid cyst tissue were subjected to polyacrylamide gel electrophoresis followed by Western blotting;To localize the distribution of OPN in pericyst, antibodies specific for OPN and macrophages (CD68) were used by immunohistochemistry staining; To appraise the relationship between the OPN and macrophages,after immunofluorescence double labelling, Image-Pro Plus 5.1 was used for colocalization of OPN and CD68; To investigate the relationship between the OPN and calcium deposits, Von Kossa and Masson's Trichrome Stains for the sequential sections were used.RESULTS: 80% cyst had the variable degree of calcium deposits, the calcium deposits concurrencely found with the OPN expression;OPN was present in all 80% of cysts by Western blotting; OPN were distributed in the side of pericystic layer directed to the parasite, macrophages were identified in the vicinity of OPN;The coexpression of OPN and CD68 was observed by immunofluorescence double labeling.CONCLUSION: The pericystic layer of hepatic hydatid cyst consists of two detachable layers with different formative mechanisms: the intra-layer(IL) directed towards the cyst of parasite was the result of...