Hypothermic Cold Machine Perfusion Preservation In Liver Transplantation

Objective Compare hypothermic cold machine perfusion (CMP) preservation with Simple Cold Storage (SCS) to investigate the model and effect of hypothermic CMP preservation in liver transplantation.Methods 35male Wistar rats were randomly divided into three groups. The livers were initially perfused without preservation in the normal group. The livers were perfused after being preserved for 12 , 24 and 36hours by Simple Cold Storage(SCS) in the control group. The livers were perfused after being preserved for 12 , 24 and 36 hours by CMP in the experiment group. The changes in tumor necrosis factor(TNF-a)content,hyaluronic acid(HA)uptake,alanine aminotransferase (ALT)activity and morphological changes in hepotocytes and sinusoidal endothelial cell (SEC) under light microscopy and electron microscopy were observed by using isolaec perfused rat liver (IPRL) model. The effect of SCS group compared with CMP preservation group.Results Compared the control group and the experiment group with the normal group ,TNF-a content and ALT activity were increased remarkably (both P<0.05)and HA uptake was decreased evidently ( P<0.05). Along with the time of liver reperfusion going on , the values of ALT, TNF-a, were significantly lower in HMP group than in control group at the same time (p<0.05). The value of HA uptake was significantly higher in CMP group than in SCS group at the same time (p<0.05).Along with the time of preservation going on, the histopathologic changes gradually aggravated, the changes included degeneration and necrosis of liver cell, different degrees configuration destroy of hepatic sinusoid in the experiment group were milder than in the control group.Conclusion These results suggest that CMP can lessen SEC injury and reperfusion injury as well as prolong preservation time effectively.