Experimental And Clinical Studies Of Complex Lysozyme On Infant Oral Candidiasis

Objective: In recent years, there was a ascending trend for fungus infection rate for a number of reasons, candida albicans is the main pathogen. Along with the high occurance rate of candidiasis, the incidence of resistance to antifungals are increasing, furthermore, antifungals had been proved to have considerable side effects. Presently, the infant oral candidiasis caused by candida albicans is usually treated by local application of antifungals, this method in one side has great side effects and not convenient for therapy, in another side, is unfavorable for the correction of the disorder of oral bacteria group.The object of this study are comprehending the clinical effects of complex lysozyme on infant oral candidiasis caused by candida albicans, evaluating the minimum inhibitory concentration (MIC) and sensitivity of complex lysozyme on candida albicans from infant mouths and exploring the relation between sensitivity of antifungal and its clinical effects.Methods: Mouth specimens of infant with oral candidiasis from the departments of stomatology of the Second Affiliated Hospital of Shanxi Medical University and the Children's Hospital of Shanxi Province were collected using throat swabs, candida albicans were separated, cultured and identified using CHROMagar chromogenic culture media.Drug sensitivity test were conducted to study the inhibitory and killing effects of complex lysozyme, fluconazole and nystatin on the separated candida albicans, the MIC values were measured using NCCLS M27-A microdilution method.The clinical study were conducted using single-blind Randomized Controlled Trials (SB-RCT), 80 infant patients were randomly divided into two groups: the treatment group were given complex lysozyme and the control group were given nystatin.Results: (1) The antifungal drug sensitivity test showed MICs of complex lysozyme, fluconazole and nystatin on the separated candida albicans were 2.03μg/ml, 3.19μg/ml and 6.03μg/ml separately; the sensitivity of candida albicans on complex lysozyme was higher than fluconazole and nystatin; (2) The clinical study showed the effective rate of complex lysozyme on infant oral candidiasis was 97.5%, much higher than the nystatin group. The therapeutic time of the complex lysozyme group was 6.46 days, much shorter than the 13.0 days of the nystatin group.Conclusion: (1) The sensitivity of candida albicans on complex lysozyme was the highest, higher than fluconazole and nystatin; (2) Application of complex lysozyme on treating infant oral candidiasis had shorter therapeutic time, higher recovery rate and less side effects. (3) The result...