| The prescription compatibility theory is the core of Chinese medical formulae. In recent years, with the development of research on the modernization of Traditional Chinese Medicine (TCM) , the study of Traditional Chinese prescription rules has not only become a pivotal aspect, but also a problem. The ideas and methods are still being studied.Insomnia is a disorder that it is difficult for the people to fall asleep and/or maintain the sleeping for more than one month. It is a common and frequent-occurring disease with high and increasing incidence in the world. Because of the addiction, western medicine is restricted to be applied in the patients with chronic insomnia. Furthermore, TCM therapy of insomnia has obvious therapeutic effect and little or no side-effect, because it is based on the Combination of Disease and Syndrome. Thus, recently, many researcher have focused on TCM study on insomnia.Jiaotai Pill is a famous formulae for treatment of insomnia of breakdown of normal physiological coordination between the heart and the kidney. It is composed of Coptis and Cassia. These two herbs harmonize to play a role in mind kidney intersects. Coptis rush down mind fire to keep up mind water.Presently, there were many documents reported concerning the theoretical and clinical studies on Jiaotai Pill, but few regarding the experimental studies. The study explored the effect of Jiaotai Pill on the autonomic activities and the contents of neurotransmitters in insomnia model rats by combination of decoction and syndrome. Firstly, the syndrome property of rats which has been given PCPA by intraperitoneal injection was explored. Secondly, the optimal proportion of the two herbs was investigated in Jiaotai Pill and established a basis of achieving a new drug for insomnia.The results of this experiment indicate that:1. The syndrome property of rats which had been given PCPA by intraperitoneal injection was Insomnia of Heart-Kidney Disharmony.1. 1 The difference of rats indicated that the model group was more active than normal group after the model group was given PCPA. The differences including circadian rhythm change and prolong time for autonomic activities. Furthmore, the rates which had been given Jiaotai Pill was more calm than those of model group.1.2 The different concentration of neurotransmitters of the rats between model group and normal group had been show that PCPA can reduced the content of 5-HT\ DA> E> NE, ACTH. However, Jiaotai Pill can enhanced the concentration of neurotransmitters, and improved the sleeping quality of rats.2. The research for optimal proportion of the two herbs in Jiaotai Pill. 2. 1 The prescription of Jiaotai Pill which the proportion of Coptis andcassia was 10:1 can calm down remarkably the rates which has been injected PCPA. It reduced the rates' duration and intension of autonomic activities, which was different from that of model group evidently. However, the prescriptions which the proportion of Coptis and cassia is 1:10 and 1:1 are noneffective to improve the rates' sleeping quality.2.2 The prescription of Jiaotai Pill which the proportion of Coptis and cassia is 10:1 increased the contain of tryptophan, noradrenalin, adrenalin, dopamine and adrenocorticotrophic hormonecorticotropin. However, the prescriptions which the proportion of Coptis and cassia 1:10 and 1:1 were noneffective to improve these indexes. It indicated that the Jiaotai Pill which the proportion of Coptis and cassia 10:1 was superior to those of 1:10 and 1:1, and maybe it exerted influence on treating insomnia from adjust the contents of neurotransmitters.In short, we consider that the syndrome property of rats which had been given PCPA was insomnia of heart-kidney disharmony. Meanwhile, the prescription of Jiaotai Pill which the proportion of Coptis and Cassia was 10:1 had the best curative effect on insomnia, and it acted on insomnia maybe through regulate the concentration of neurotransmitters. |
PDF Full Text Request