The Activiation Of MAPK P38 Of PBMCs In HBV Infected Patients Being Stimulated By LPS In Vitro

[Objective] To observe the baseline level of MAPK p38 (Total and pTpY180/182) in the PBMC of HBV infected and the level after stimulated by LPS, and estate the relationship of MAPK p38 with the HBV duplications and inflammation[Methods] ①The experimental samples were selected from out-patients and in-patients in the department of infectious disease of the first affiliated hospital of Kunming Medical College. The total were 40. The patients were divided into four groups according to HBV serum antigen and antibody, the level of HBV-DNA and ALT. A group: HBsAg(+), HBeAb(+), HBcAb(+), HBV-DNA≥ 1.0E04 (e~- DNA(+) group) 8 cases; B group: HBsAg(+), HBeAb(+), HBcAb(+), HBV-DNA<1.0E04 (e~- DNA(-)group) 8 cases ,ALT<40U/L; C group: HBsAg(+), HBeAg(+), HBcAb(+), HBV-DNA≥ 1.0E04,ALT<40U/L (e~+ ALT<40U/L group) 7cases; D group: HBsAg(+), HBeAg(+), HBcAb(+), HBV-DNA≥1.0E04, ALT>40U/L (e~+ ALT>40U/L group) 9 cases; E group: the persons without HBV infected, control group , 8cases. ②The peripheral blood mononuclear cells were separated from human venous blood . The cells were divided into two groups. The baseline level of p38(Total) andp38(pTpY180/182) were detected with one;the other was dected after being stimulated by LPS. The p38(Total) and p38(pTpY180/182) contents were detected with quantitive ELISA.[Results]The baseline level of p38(Total) and p38(pTpY180/182) has no statistical difference before being stimulated by LPS (ANOVA anlysis, p>0. 05). The level of p38(Total) has no statistical difference during each group after being stimulated by LPS (ANOVA anlysis, p>0.05) . Both e~+ ALT>40U/L group vs control group and e~+ ALT>40U/L group vs e~- DNA(-) group...