The Study Of The Relationship Between APOE Gene Polymorphism And Atherosclerotic Cerebral Infarction

Objectives To investigate the relationship between apolipoprotein E (ApoE) gene polymorphism and atherosclerotic cerebral infarction (ACI) in Kunming Han subjects, and analyzed the frequency distribution of APOE alleles in ACI to assess whether there is any difference between young and middle-aged and elderly patients,and study the character of the concentration of ApoE in patients with ACI.Methods Polymerase chain reaction-restricted fragments lengthpolymorphism (PCR-RFLP) was used to determine ApoE genotype on 142 Kunming Han subjects including 92 cases with ACI including 42 young and middle-aged cases <60 years of age and 50 elderly cases ≥60 years of age and on 50 age and sex-mathed healthy controls.And the concentration of ApoE in patients with ACI and in control subjects was determined.Results The frequencies of ApoE 3/4 genotype and epsilon4 allele werehigher in patients with ACI than in healthy controls . There was significant statistical difference either in genotype or in allele frequency between the two groups (P<0.05) and no statistical difference between young and middle-aged and elderly patients with ACI (P>0.05) . There was no statistical difference in the concentration of ApoE in the groups (P>0.05) .Conclusions Our data show an association between APOE gene polymorphism and the onset of ACI and support the possibility that epsilon4 allele is a susceptibility locus for the risk of ACI. The frequency distribution of APOE alleles in ACI has no any difference between young and middle-aged and elderly patients.