Three-dimensional Endothelial Cell Culture In Vitro

ObjectiveAngiogenesis is fundamental process by which new blood vessels are formed. It is essential in the development,invasion and metastasis of tumors. There has been many methods of three-dimensional endothelial cell culture,which provide a new thoughtway for the methods of tumor-therapy. The intention of this research is to study the difference of two methods of three-dimensional endothelial cell culture in vitro and to provide experimental support for the discovery of new ways of tumor-therapy.Methods1.Fill trypsin-EDTA into human umbilical vein to obtain sing-cell mixing suspension.2.Plate cells to the cultivate plate repeatingly to obtain purified HUVECs.3.Identifying HUVECs by the technique of immunohistochemistry as CD31,CD34 and factorâ…§is reference.4.In vitro three-dimensional culture of endothelial cell.4.1 In vitro three-dimensional cluture of endothelial cell in the surface culture system.4.2 In vitro three-dimensional culture of endothelial cell in the mixing culture system.5.Comparison of two methods of three-dimensional endothelial cell culture by statistic analysis of the experimental results.Result1.Single-cell mixing suspension most of which are HUVECs is obtained by filling trypsin-EDTA into human umbilical vein.2.HUVECs at the purity of 99.5% are obtained by plating cells to the...