Rat Depressive Model Behavior Trends Made By Chronic Stress And Their Level Of Cytokines

Objective Depression is a multifaceted psychological disorder that involves changes in behavior, immunological function and responses. Make Wistar rat depressive model by chronic stress which can not be forecasted and isolate feed. Observe the weight, sucrose preference solution, automatic behavior and Morris maze. Analyze all of the data from the experiment. Measure the level of serum cytokines of the rat model, such as IL-2, TNF-α, IFN-γ,IL-4 and IL-6. Observe the correlation between cytokines and weight, sucrose preference and behavior. Discuss the role of cytokines playing in the depression.Methods Thirty Wistar rats (200~220g) from ShanDong University animal center were used. Rats were allowed 1 week to acclimate to the surroundings before beginning any experimentation. After 7 days normal feeding, all animals were randomly separated into 3 groups, one is normal control group, one is stress group and the last is treated group by paroxetine. There was no any stress to normal control group which was fed resident. Seven stress repeat 3 times put on the stress group and this group were individually housed. The third group, which is treated by paroxetine, got the same stress as the second group and given the therapy at the same time. Measure the rats' weight, sucrose preference, automatic behavior and Morris maze including total time, swimming time in the center, average speed and cross number, then analyze the influence of stress to rats and the effect of paroxetine. After 3 times stresses, the levels of serum cytokines were measured and analyzed the correlation between cytokines and weight, sucrose preference, automatic behavior.Results1.There were no differences among weight, sucrose preference,...