The Relationship Between TNF-α Gene Polymor Phism And Alcoholic Liver Disease In Korean And Han Men Of Yanbian Area

[Objective]: To investigate the genotype and the allele frequency of Tumor necrosis factor-alpha(TNF-α )-238, -308 in peripheral blood mononuclear cells(PBMCs) in Korean and Han men of yanbian area and the relationship between alcoholic liver disease (ALD).[Methods]: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect TNF- α -238, -308 single nucleotide gene polymorphism in the health control group (Korean50,Han45), alcohol dependent group (Korean56,Han45) and ALD group(Korean 58,Han53) in Korean men and Han men of Yanbian area, calculated and compared the genotype and the allele frequency of three groups.[Results]: In Korean men the genotype frequency of TNF-α -238G/A has no significant difference between alcohol dependent group (3.45%) , ALD group (3.57%) and health control group (2.0%)(p>0.05). No the genotype of TNF-A/A was detected in all the samples. The genotype frequency of TNF- α -308G/A has no significant difference between alcohol dependent group (12.07%) , ALD group (12.5%) and health control group (12.0%)(p>0.05). No the genotype of TNF-A/A was detected in all the samples.In Han men the genotype frequency of TNF- α -238 has no significant difference between alcohol dependent group(2.22%)N ALD group(3.77%) , and health control group (2.22%)(p>0.05) .No the genotype of TNF-A/A was detected in all the samples. The genotype frequency of TNF- α -308 has no significant difference between alcohol...