| Objective: The purpose of this study is to investigate the effect of TGP in hepatic ischemia and reperfusion in rats.Method: Hepatic ischemia and reperfusion medel in 60 SD rats was made by hepatic veins and artery reperfusion for 60 minutes following hepatic portal vein and artery occlusion for 30 minutes. 60 rats were divided into six groups randomly. In group A (control group, n=10), rats were not subjected to ischemia and no treatment was given. In group B (I/R injury group, n=10), rats were subjected to 30 minutes ischemia followed by reperfusion for 60 minutes. In group C (SOD-treated group, n=10), SOD was given (500U/kg, i.v.) before laparotomy. In group D1 (TGP-treated group, 60mg/kg·d, n=10), group D2 (TGP-treated group, 120mg/kg·d, n=10) and group D3 (TGP-treated group, 240mg/kg·d, n=10), TGP was administered intragastrically, ig., qd, for 4d. Serum alanine aminotransferase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) and tumor necrosis factor-α (TNF-α) were determined. Liver tissues were taken for determination of malondialdehyde (MDA) levels, superoxide dismutase (SOD) levels, glutathione (GSH) levels, nitrite oxide (NO). Expression of inducible NO synthase (iNOS) and nuclear factor-κBp65 (NF-κBp65) were evaluated immunohistochemistry.Results: At 1h of reperfusion, ALT, AST and TNF-α in group C, D1, D2, D3 decreased compared with group B (P<0.05), and there were not significant differences... |
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