The Effects And Mechanisms Of Total Glucosides Of Paeony On Immunological Hepatic Fibrosis With Rats

Objective To study the effects and mechanisms of total glucosides ofpaeony (TGP) on immunological hepatic fibrosis induced by human albumin in rats . Methods The model of immunological hepatic fibrosis induced by human albumin was prepared. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) , prolidase (PLD), nitric oxide(NO) in serum, hydroxyproline(Hyp) content, malondiadehyde(MDA) content, superoxide dismutase(SOD) and glutathione peroxidase(GSH-px) activities in liver homogenate were assayed by spectrophotometry; Serum procollagen type III (PC III) and hyaluronic acid (HA) was determined by radioimmunoassays. The level of transforming growth factor- (TGF- )was determined by ELISA method. Hepatic tissue sections were stained with hematoxylin and eosin and examined under a light microscope. Results: Histological results showed that TGP improved the human albumin -induced alterations in the liver structure, alleviated lobular necrosis and significantly lowered collagen content. The anti-fibrotic effect of TGP also confirmed by decreased serum content of HA and PCIII in TGP-treated group. Moreover, the treatment with TGP effectively reduced the hydroxyproline content in liver homogenate. However, the level of ALT and AST increased in fibrotic rat but had no significance compared with normal control, whereas the ratio of A/G decreased also had no significance. TGP had no effect on level of ALT ,AST and the ratio of A/G Furthermore, TGP treatment significant blocked the increase in MDA and NO, associated with a partially elevation in liver total antioxidant capacity including SOD and GSH-px. Meanwhile, TGP treatment significant decreased the production of TGF- 3 .Conclusion TGP have beneficial effects on hepatic fibrosis in rats by inhibition of collagen synthesis, decreasing oxidative stress and TGF- production.