Risk Factors Of Crohn's Disease And NOD2/CARD15 Gene Polymorphisms: A Case-control Study

[BACKGROUND]Crohn'disease (CD) and ulcerative colitis (UC) are common clinical subtypes of idiopathic inflammatory bowel disease (IBD) characterized by chronic inflammation of the gastrointestinal tract.CD patients may present with chronic abdominal pain and diarrhea.Patients with CD can have potentially severe complications such as intestine congestion,perforation.CD was generally considered very common disease in Europe and America.However, the incidence of CD is rising even in Chinese individuals over the last 20 years. Because CD is so severe and costly that an all-round study is very important. The precise aetiology of the disease is unknown,but an interplay of environmental risk factors and immunologic changes will trigger the onset of the disease in a genetically susceptible host.[AIMS]To explore the potential relevant risk factors of CD. These factors are principally made up of the factors related to family condition and work ,the factors related to individual usual practice, the factors related to disease history, mentality and drug ,the factors related to perinatal events and early infection ,the factors related to diets, the factors related to smoking and wine-drinking, To examine a possible association between alteration in the NOD2/CARD15 gene for the three major genetic substitutions (R702W,G908R,and 3020inC ) with CD.[METHODS]The study design project is a case-control (1:M=1:4),whose matched factors are age and sex.The cases and the control populations are both based on hospital .The cases were from 51 patients of Suzhou municipal hospital. 204 controls also were from Suzhou municipal hospital. Blood samples of 20 cases and 40 controls were conducted the RTLF-PCR to determine susceptibility of CD from the role of genetic polymorphisms. Our work spanned from Oct 2002 to Apr 2006. Quality control measures were used, such as preliminary experiment ordering to control concordance rate and Kappa between 80%-85%,repeating and affirming data to control bias, repeated measuring RTLF results in 10% samples, and double data entry. Odds ratio and 95% confidence interval were used to estimate the association between risk factors and CD. Major statistical methods were used such asχ2 test and Logistic...