The Investigation Of Vitrified Mice Blastocysts

Background and ObjectivesThe cryopreservation of embryos is indispensable and important in IVF technology, as controlled ovarian hyperstimulation usually leads to extra embryos which are not transferred (the embryo transfer 2-3 a number) in vitro fertilization-embryo transfer (IVF-ET) cycle. Most of the literature on prospective randomized study study reports, transfer of blastocyst-stage embryos carries a significantly higher implantation rate and pregnancy rate compared with embryos at early cleavage stages. Blastocysts transfer pregnancies and the availability of higher implantation rate. With Blastocysts transfer development, blastocysts freezing is imperative. The survival of blastocysts cryopreserved by slow freezing was quite low, Vitrification, by contrast, enables rapid cooling of samples by direct plunging into liquid nitrogen from temperatures above 0°C without ice forming even in the extra-cellular medium, by use of a very high concentration of cryoprotectant, so that survival improved. In vitrification, the chance of intracellular ice forming can be minimized, but toxic effect of cryoprotectant on the cell becomes a great obstacle. Most of vitrification used by straws in the past, this approach limited the cooling rate. It can be obtained ultra-rapid cooling and warming rate by modified vitrification methods, which improved the structure of freezing carrier, reduced volume of...