Study Of The Cryopreservation Of Mice And Human 2～8 Cells Embryos By The Vitrification Method

Objective: Study on the cryoprotective effect of the EDS-40vitrification solutions to mice and human 2~8 cells embryos Methods: Design EDS-40 vitrification solution. According to randomgroup doctine, the test is divided into experiment group and contrast group.Experiment group(a) use EDS-40 vitrification solution, contrast group(b)use EFS-40 solution which is considered the best vitrification. contrastgroup(c) use program freezing method.1.Vitrification test of EDS-40solution. 2.Mice embryo freezing: In group a and b good kunnming mice2～8 cells embryos were being vitrified in EFS-40 and EDS-40 solutions at25℃,after packaged in 0.25ml plastic straws ,then embryos were immersedinto liquid nitrogen. In group c mice 2~8 cells embryos werecryopresevered in program freezing equipment. All samples werecryopreserved about 2-3 months. The straws were warmed rapidly in 25℃water, and agitated gently, then embryos were transferred into Ham'sF12medium and cultured. Then watch its survival rate and its reexplanded rates.Software SPSS11.5 analysis was used to determine differences inpercentage effect. 3.Human embryo freezing: Human embryos werecollected from our center,the cryopreserved,rewarming and statisticalanalysis methods is same to animal experiment . Result:1.EDS-40 solution were vitrified.2. The survival rate of mice2～8 cells embryos were 84.31%,77.98%,69.23%;the reexplanded rateof mice 2～8 cells embryos were 79.41%,66.97%,53.85 %。3.Tthesurvival rate of human 2～8 cells embryos were 53.85%,68%,57.14%;the reexplanded rate of human 2～8 cells embryos were 26.9%,56%,28.57%。 Conclusion: 1. EDS-40 vitrification can be verified and can be thecryoprotectant to vitrifiy embryos. 2. The effect of EDS-40 solution isbeteer than EFS-40 and program freezing method on cryopreserving mice2～8 cells embryos. 3. The effect of EDS-40 solution is lest than EFS-40and is similar to program freezing method on cryopreserving human 2～8cells embryos,besides the species difference ,the cause is related to thequality of embryos our selected,and need continue to study.4. Theexperiment outcome manifest the effect of vitrification is better thanprogram freezing method.