Study On Drug Sensibility Of Bovine LECs' Sensibility In Differernt Growth State In Vitro

Extracapsule cataract extraction(ECCE) and introcular Lens (IOL) implantation play the main role in cataract surgery. Posterior capsule opacification (PCO), the major complication after ECCE, will cause the loss of the patient's visual acuity seriously. The proliferation, differentiation and apoptosis of Lens epithelial cells(LECs) has a close relationship with PCO, therefore, inhibition of the proliferation, differentiation and apoptosis of BLECs by using drugs is one of most important means of preventing or reducing the development of PCO.Different kind of drugs has different side effects in vivo. For example, antimitotic-antimetabolites such as mitomycin-C (MMC) and 5-fluorouracil (5-FU). Although it shows promising effect in preventing the proliferation, differentiation and apoptosis of BLECs, it has limited uses because of its significant side effects-having toxicity to the corneal endothelial cells. On the other hand, nontoxic or the least harmful agents such as dexamethasone and heparin have little side-effects when used in patient's eyes, so they can be used to prevent the proliferation, differentiation and apoptosis of BLECs. To study the intraocular toxicity, we often select some concentration of drugs to inject into anterior chambers of whit rabbits. But this has its defect: there are great individual differences in white rabbits. Thus the result of animal experiments is not so precise.Purpose of this experiment is to find some method to make up the defect of animal experiment. The growth state of BLECs cultured in vitro can be influence by...