An Experimental Research Of Trifluoperazine On The Proliferation Of Human Lens Epithelial Cells In Vitro

[Objective] : To investigate the effects of trifluoperazine on the proliferation of human lens epithelial cells in vitro and it's possible mechanism of action. And search for a new drug to treat the after cataract.[Materials and Method] : The human lens epithelial cells were given from the Cell Center of Beijing . The third generation cells were used to be treated with different concentrations of TFP (2μg/ml, 4μg/ml, 6μg/ml, 8μg/ml); culture media only(control group) .Cell proliferation was determined by two different methods : 1 MTT assay : Cells were cultured in 96 well plates for 24h, 48h, 72h, 96h and measured the value of optical density respectively. 2 FCM analysis : Cells were placed in 6 well plates for 24h and the changes of cell cycle were measured.[Results] : 1 There were no significant difference of proliferation inhibition between control groups and groups treated with TFP of 2μg/ml in 24 hours. But after 48 hours, the lens epithelial cells were significantly inhibited(P〈0.01);when the TFP concentration up to 4μg/ml, the inhibiting effect was occurrence in 24 hours ,The result shown significant difference comparing to control group(P〈0.01).Within 48 hours, the inhibiting rate of each groups was raised with increase of the TFP concentrations(P<0.05); In 72h, the inhibiting rate of 4~6μg/ml concentrations was raised with increase of time(P<0.05).When the concentration reaches 8μg/ml, there was no statistical difference in the inhibiting rate of different times.2 FCM analysis Showed that in TFP group the rate of the G0/G1-phase cells increased, but S + G2/M-phase cells decreased. The results showed that TFP block the cells in G0/G1 stage. [Conclusion] : TFP can inhibit the growth of human lens epithelial cells in vitro and the inhibition were shown to be time and dose dependent. Study about TFP provides an experimental basis in treating posterior capsular opacification.