The Effect Of Solanum Lyratum Thunb Compound Preparation To The Apoptosis Of Wound Healing And Related Gene Proteins Of Rats Skin Cells

Apoptosis becoming a hot theme in the study of wound healing. As apoptosis in not only important to the normal wound healing but also important to the abnormal wound healing such as scar and so on.We studied the rats skin wound. Chose 32 rats, make wound on 24 rats, each rat made 3 wounds. The two wounds of the right is example group the left is contrast group. The rats were sacrificed in 5 h, 1 d, 3d, 5 d, 7 d, 10 d, 15 d, 20 d after treatment and tissues of the skin wound were observed the morphological changes through H.E stain. Apoptoic DNA of skin cells were extracted from the wound, then detected apoptotic DNA ladder formation by agarose gel elestrophoresis and apoptosis rate of skin cells by TUNEL method quantity. The immunohistochemistry method was adopted to measure and locate the expression changes of gene proteins—bcl-2 and caspase-3 to the inhibition of apoptosis. Observed the wound of the rest rats and measured it.The results showed that Solanum Lyratum Thunb group healed faster the contrast group. The apoptosis and expression of Bcl-2 and caspase-3 were performed on rats skin incised wounds at different posttraumatic interval by immunohistochemical technique.The conclusion showed that apoptosis exists in the wound healing, Solanum Lyratum Thunb has some effect to induce the wound healing and both bcl-2 andcaspase-3 could control skin wound cells apoptosis.