Initial Research On Pharmacodynamics And Mechanism Of A New Traditional Chinese Drug F-GC In Diabetes Mellitus' Prevention And Treatment

F-GC, extracted from brown algae, is Fucoidan-Glycocalyx Compound which is a kind of proteoglycan with sulfate group. It has been demonstrated that as a potent anti-oxidant , it could modulate oxidative stress , lower blood glucoe and blood fat, regulate organism immunity and strengthen physical fitness.In this paper, we have studied the effect of F-GC on Diabetes mellitus'prevention and treatment, also investigate the mechanism of action of F-GC.To begin with, we set up appropriate diabetic model, Blood glucose, plasma insulin concentration and glycogen in liver were observed after fed with F-GC 30 days incessantly. We found that F-GC could lower blood glucose of diabetic mice and promote glucose uptake and utilization of liver cells to some extent; There weren't differences in the insulin content among each groups . F-GC could lower TG content; By the analysis of pathyology slices picture , we found F-GC also could restoreβcell of islet of diabetic mice and promoteβcell function to some extent. This experiment also indicated : compared with diabetic model group , the activity of SOD of liver and kidney in F-GC groups increased remarkably, the activity of GSH-px and CAT in kidney also elevated significantly. In liver, the activity of GSH-px changed a little. So Antioxidative capacity in liver and kidney was strengthened.Then, in order to investigate the effects and mechanism of F-GC deeply, we carried out some experiments at cellular level with C2C12. We found that F-GC dose groups couldn't promote glucose uptake and utilization of C2C12 on physiological condition. When utilized with insulin, F-GC could promote glucose uptake and utilization of C2C12 , The effect was more significant than insulin alone, so we think F-GC could encourage insulin sensitivity. Oxidative stress can be induced when H2O2 was utilized to stimulate C2C12 at different level, under this condition , F-GC could relieve oxidative damage to some extent and promote glucose uptake, F-GC also could promote proliferation and differentiation of C2C12 . oxidative stress could slow the flowability of C2C12 membrane so that it couldn't function normally. F-GC could relieve oxidative damage to membrane to some extent, protect membrane and promote restoration of function; F-GC could lower the ROS content of C2C12 which were in oxidative stress status. NF-кB is very sensitive to oxidative stress, it can be actived and express in oxidative stress status. This experiment proved that the expression of NF-кB increased after C2C12 were stimulated by H2O2. F-GC could relieve the oxidative stress and decrease the expression content of NF-кB in C2C12 so that some genes which are intimately related to the occurrence and development of diabetic mellitus and complications couldn't express.