Investigation Of The Effect Of Chinese Medicines On The Growth Of Mouse Hair Follicles In Vitro

ObjectiveWe established an in vitro organ culture model of mouse follicles andinvestigated the efFect of water soluble extracts of traditional Chinese medicines on the growth of mouse hair follicles. We also established an in vitro model of hair follicle bulb cell culture and observed the efFect of water soluble extracts of traditional Chinese medicines on the proliferation of mouse hair follicle bulb cells.Materials and MethodsFour-day-old C57BL/6J and 4 w to 6 w old C57BL/6J mice were offered by the experimentation animal centre of Zhejiang University.Carthamus tinctorius, Angelica and Biotae were offered by Basic Department of Zhejiang Chinese Medicine Research Institute.Mouse vibrissa follicles were isolated by microdissection and cultured in Hair follicle nutrient liquid with(experimental groups) or without (control group) water soluble extracts of Chinese medicines.The hair growth lengthin vitro organ culture with or without Chinese medicines at different concentrations were observed.he dorsal hair follicle bulb cells of 4 days old C57BL/6 mice were also cultured in hair follicle nutrient liquid with(experimental groups) or without (control group) water soluble extracts of Chinese medicines. MTT were used to investgate the effects of water oluble extracts of Chinese medicines on proliferation of hair follicle bulb cells.ResultsOn day 7 of culture ,we observed a higher rate of the mixture groups at concentration of 1.5 X10'5 g/m K l.SXlO^g/mK 1.5X10'7g/ml than thatin the control group (P<0.05). On day 3 and 5 of culture, we also observed a higher rate of the cell growth activity in the mixture groups than that in the control group (P <0.05). Howeverdjthere were no distinct different of the hair follicle growth and the cells growth activity between the single medicine groups and the control group. And the mixture groups at over low(l.5X10"8g/ml)or superhigh( 1.5X10*4g/m 1 ) concentration had no effects on the hair follicle growth and cell proliferation as well.ConclusionsMitxture of some water soluble extracts of traditional Chinese medicines can promote the growth of mouse hair follicle in vitro and stimulate the proliferation of hair bulb cells. While water soluble extracts of a single traditional Chinese medicines have no effect on the growth mouse hair follicles and hair bulb cells.