The Expression And Intervention Of Rosiglitazone Of Insulin Receptor, Insulin Receptor Substrate-1 And Glucose Transporter-4 Of Chronic Pancreatitis Rats' Striated Muscle Cells

Objective: To establish a reliable chronic pancreatitis rat model and observe its pathological and ultrastructural changes, so as to research of etiology of chronic pancreatitis and evaluate of the therapeutic effects. Methods: Healthy SD rats were selected and separated into 2 groups by random: model group and sham operation group. The rats of model group were injected of oleic acid into the rat pancreatic duct using microsyringe to induce chronic pancreatitis. The pathological changes of pancreas were investigated over a period of 6 weeks after induction of this model. Results: Histologically, the widening of acinar lumen and cellular vacuolization occurred within 24h at the parenchyma neighboring the small ducts filled with the injected solution. Degenerative parenchyma, interstitial edema and inflammatory cell infiltration were pronounced 1 week later. Thereafter, duct-like tubular complex formation progressed and the exocrine tissue exhibited marked atrophy of the gland with irregular fibrosis and fat replacement after 6 weeks. Meanwhile, endocrine tissue showed overt damage. Conclusion: The chronic pancreatitis rat model has fine reliability, stability and high repeatability. The model rat's pathological changes of pancreas were similar with human chronic pancreatitis. It provides a good experimental model for research of etiology of chronic pancreatitis and evaluate of the therapeutic effects.