The Effect Of Fungus Pathogenesis Of Surgical Infection On The Formation Of Hypertrophic Scar In Rabbits Ears

Objective: To study the effect of fungus Pathogenesis of surgical infection on the formation of hypertrophic scar in rabbits ears after injury. Methods: Full-thickness excisional wounds and 1cm in diameter were created over the ventral surface of 12 adult New Zealand white rabbits ears which were randomly divided into fungus Pathogenesis(group A), Staphylococcus aureus group(group B), blank group(group C) and were observed wound healing time of group A, B , C and incidence of forms of excessive scar.The change of fibroblasts in forms of excessive scar were investatigated in different time thorough histomorphometric, TGF-β₁ and collagen- I in forms of excessive scar were tested in different time through immunohistochemistry. Results: Excessive dermal scars on rabbits ears are similar to human hypertrophic scar in Candida albicans group and Staphylococcus aureus group. The mean values of wound healing time of groupA, B, C was 14.7±1.0, 15.3±1.1, 11.2±1.3 days respectively, which have statistical differecnce. The incidence of excessive dermal scar is 85.4% in Candida albicans group and 91.6% in Staphylococcus aureusgroup, both the two groups were found to have no statistical differecnce, but have statistical differecnce with the blank control group; The number of proliferative fibroblasts of the Candida albicans group are the most among three groups in the postoperation 28daysN 49days> 60days. the express of TGF- P i and collagen-1 between Candida albicans group and Staphylococcus aureus group have no statistical differecnce in the postoperation 28days> 60days,but have statistical differecnce in the postoperation 49days; both Candida albicans group and the blank control group have statistical differecnce in the postoperation 28days^ 49days^ 60days. The number of fibroblast and the express of TGF- 0 i and collagen- I in all groups decrease along with the time course. Conclusion: Candida albicans of surgical infection enlarge healing time and increase forms of excessive scar incidence in rabbits ears-, The number of fibroblast ?. the express of TGF- P i and collagen-1 in healed excisional wounds of rabbits ears are higher than normal tissues; further proving the animal model of rabbit ear for hypertrophic scar is stable and repeated experiment method for study of the human hypertrophic scar. To apply the hypertrophic scar animal model which was contanminated by Candida albicans in rabbits ears after injury will be helpful to study the formation mechanism of human hypertrophic scar.