Research On The Integrative Use Of The Cordyceps Medium

The research on extraction and purification technique, examination method,construction identification of cordycepin and exopolysaccharide in the solid of Cordyceps Militarism Link,as well as exopolysaccharide antioxidation activity and the two fermentatin methods, had been done indepthly and systematically, the result are satisfactory.1. Through study on solid and liquid ferment we found that the highest content of cordycepin is seventh and eighth day in mycelia and liquid culture media respectively by HPLC in liquid ferment; in solid ferment forty days as a periods is god for mycelia growth.2. The optimal technique parameter of extracting cordycepin is researched by orthogonal design. The result is that the best extractive condition of cordycepin was use ultrasonic extraction three times in twenty times of 75% ethanol solution,and 40 mins each time.3. The optimal technique parameter of extracting exopolysaccharide is researched by orthogonal design. The result that The best extractive condition of exopolysaccharide was extracted three times in twenty times of water,and 90 mins each time.4. According to the optimal technique of extracting cordycepin and exopolysaccharide. The studies on the method of extraction cordycepin and exopolysaccharide at the same time had done. Respectively.cordycepin and exopolysaccharide have been determined by HPLC and phenol-sulfuric acid method ; it is the different content in cordycepin and exopolysaccharide through the different purification methods.5. In the different artificial Cordyceps Militarism Link, the content of cordycepin is different and obvious by the measurement of RP-HPLC, the highest is shannxi jingyang Cordyceps Militarism, xiuzhou qimiao No.2 is secondly, the content of cordycepin is more high in mycelia than in the culture media in the same kind of Cordyceps Militarism.6. The crystallize II have been characterized by TLC,HPLC, Melting Point Determine,IR, and chemical element analysis, the result is in accordance with the cordycepin spectrum data.7. Studying 15%,30%,50%,70% four different ethanol density exopolysaccharide antioxidize activity.the result showed that all of the four-part have antioxidize activity .but the 30% parts is the most strong.