The Immunohistochemistry Study Of TNF-α And Its Receptors In Pallium Of Rat Brain Following Whole-brain Irradiation

Objective: To investigate the possible role of acute phage of brain radiation injuries by the observation of the dynamic expression of the TNF-α , TNFR1 and TNFR2 in the rat pallium.Methods: A early model of brain radiation-induced injuries in the rat was developed. Analysis of the expression of TNF- α TNFR1 and TNFR2 in the pallium was done at different dose and time level after whole-brain irradiation with immunohistochemistry and computer technology.Results: The expression of TNF- α , TNFR1 and TNFR2 in the pallium was at lower level in OGy group. There wasnt significant difference between the expression of normal group and OGy group (P>0.05) . The expression of TNF- α was observed at 2 Gy 10 Gy and 30 Gy groups and different time levels with microscope. .The expression of TNF- α reached the peak level at 12h in those three groups (P<0.05 ) ; the expression of TNF- α in 30Gy group was the highest, but there wasn't significant difference between the 30Gy group and the 10Gy group (P>0.05 ) . The expression of TNF- α in those three irradiated groups returned to the basic level after 24h. The dynamic expressions of TNFR1 and TNFR2 were similar to that of TNF- α bythe same way, but the positive number of TNFRl and TNFR2 was lower than the latter.Conclusion: The expression of TNF- a ? TNFRl and TNFR2 in the rat pallium following radiation induced injuries increases and decreases rapidly within 24h and shows that they may play a important role in the early pathological processes of the ionizing irradiation injury of rat brain.