Expressing And Shedding Of Tumor Necrosis Factor Receptors In Digestive Neoplasm

Objective: Digestive neoplasm is a serious threat to human health. Its untypical clinical symptoms made it not easy to be detected in early stage and made it some difficult to diagnose early and treat in time. The common way now used to detect neoplasm is mass health checkup, which including physical examination, such as endoscope~ B ultrasound and CT; and biochemical test, like enzyme and tumor marker. It had been agreed widely that tumor necrosis factor (TNF) had certain cytotoxic effect on tumor cells, but there is not enough research on the expression of tumor necrosis factor receptors (TNFRs) and soluble tumor necrosis factor receptors (sTNFR5) in tumor, especially the effect of thermotherapy and radiotherapy on sTNFRs in hepatocellular carcinoma ([ICC) has not been reported systematically and detailedly. To further invetigate the abnormal immune status and to discuss the effect of TNFRs-sTNFRs system in digestive neoplasm , a series sentitic studies were expanded. Methods: (1) Enzyme-linked immunosorbent assay (ELISA) was used to assess the levels of sTNFRs in serum and ascitic fluid and is also used to check the changes of sTNFRs before and after mutipolar autocontrol radiofrequency thermal ablation (MARFTA). At the same time ELISA was used to assess the concentration of sTNFRs in culture supertanents of PBMCs and tumor cell lines. (2) Flow cytometry (FCM) was used to study the peripheral blood mononuclear cell (PBMC) TNFRs expression. (3) lmmunoliistochemical method was used to examine the expression of TNFRs in 91 cases of colorectal carcinoma (CRC), 51 cases of gastric neoplasm and 15 cases of I-ICC and as well tumor cell lines expression before and afIer X-ray i tradiation. (4) A human hepatocarcinoma cell line HepG2 and human colorectal carcinoma cell line Lovo were used to evaluate the effect of 5 Gy X-ray irradiation. The -5- apoptotic ratio of cell lines after 5 Gy X-ray irradiation were determined by means of hematoxyl in-eiosin(J-IE) staining and flow cytometry(FCM). The expression levels of TNFR-p55 and TNFR-p75 before and after exposure of the two cell lines were investigated by imrnunohistochemical methods. Results: (I) The levels of sTNFRs in serum and ascitic fluid of the i-ICC patients were obviously higher than the levies of sTNFRs in serum of the controls. And there was a significant correlation between the levels of sTNFRs in serum and that in ascitic fluid of the i-ICC patients . The levels of sTNFR-p75 in serum and ascitic fluid were higher than the levels of sTNFR-p55. The levels of sTNFRs decreased significantly after MARFTA. (2) The 11CC group and the control group both were divided into three subgroups: the first of which was co-cultured with IL-2; the second subgroup was co-cultured with nothing; and the third subgroup was not cultured and the TNFRs in PBMC was measured directly. The result showed that the expression ratio of TNFRs in the first subgroup was significantly higher than that of the second and the third subgroup (P