Animal Research On The Effect Of "Membrane Of Interlaced Sodium Hyaluronate" In Preventing Peritendon Adhesion

Objective : To explore the effect of "membrane of interlaced sodium hyaluronate "in preventing peritendon adhesion through animal experiment;Methods: Classified 24 rabbits into group of A and B and C randomly, had the lateral tendon of rabbit's soleus muscle traumatized with ameliorated Rothkopf and Perterson methods and adopted a self-control design to research . After wrapping the left injured tendon with membrane while treating the other side with saline water, we observed the effect of the membrane in preventing adhesion by various methods and experimental apparatus such as pathology observation , Sirius red and picric special dying and electronic microscope when the rabbits were putted to death according to experimental schedule.;Results: the experimental tendons acquired a fair cicatrisation and the adhesion obviously was less than the control tendons. The adhesion grading result indicated a significant difference between the experimental and control tendons(A and C group P < 0.01 ,? B group P < 0.05); Pathology observation indicated that the tendons draped with sodium hyaluronate suffered a slighter inflammatory response compared to the tendons treated with saline water. And moreover the fibers in the experimental group were well organized nevertheless a disorder of fiber arrange in the opposite group as a control. From the slices sampled 4 weeks later we could see a significant difference between ratios of collagen fibers I /III from the contrastive sides withSirius red and picric method; 2 weeks after the experiment the electronic microscope indicated the proliferating extent of collagen fiber cells significantly prevailed over the control side; 6 weeks later the experimental fibers were loosely and trimly arranged while the control fibers compacted closely and intercrossed each other. Conclusion: The effect of the "membrane of interlaced sodium hyaluronate " in preventing peritendon adhesion is quite dependable as we assumed beforehand. The internal mechanism plays the most important role in the cicatrizing process of experimental side while the both internal and external mechanism worked collaboratively together in the control side. For the merits of good biological compatibility and endurable persistence in body, this newly developed material should be given consideration of application in clinical cases. However the following defects of this kind of membrane are still waiting for practical solutions (l)brittle quality (2) terrible adhesive character toward to neiboring tissues which could lead to a costless waste .(3)expansive metamorphosis would produce stress on the healthy tissues (4) sclerotic surface of the tendon after the process of reconstruction and rehabilitation (5) uncertain effect against the adhesion of other organs treated with surgery operation...