Regulation Of Adipocytokines In Dyslipidemic Mice By Mortierella Isabelina

Polyunsaturated fatty acids (PUFAs) are essential fatty acids for human life, and play pivotal physiological roles in obesity, diabetes, cardiovascular disease and cancer. The amount of study has suggested PUFAs have hypoglycemic and hypolipidemic effects by regulation of gene expression. In traditional view, adipose tissue is considered as a passive reservoir of energy storage. The discovery of leptin has focused attention on the role of cytokines secreted by adipose tissue, which is a complex, highly active metabolic and endocrine organ. A better understanding of adipose tissue in obesity and insulin resistance will likely permit rational approaches to treat obesity and type II diabetes.Mortierella isabelina is a filamentous fungi riched in linoleic acid and linolenic acid. This fungi has been shown to positively affect blood lipids and glucose. However, molecular mechanism of their effects on metabolic disease are unclear. In this study, we established diet-induced dyslipidemic and type II diabetic model to study the effects of Mortierella isabelina on the adipose tissue-derived cytokines, such as leptin, adiponectin, resistin, TNF-alpha. The result indicated that i) after 7-weeks administration of Mortierella isabelina, high fat diet-induced dyslipidemic mice led to body weight-loss, decreased epidydimal and perirenal white adipose tissue mass however no difference in interscapular brown white adipose tissue, reduced serum level of total cholesterol (TC), triglyceride (TG) and glucose, increased HDL-C level as well.ii) serum level of TC, TG and glucose were reduced in diabetic mice by administration of Mortierella isabelina and its PUFAs extraction, albeit no significantly difference. To further understand if Mortierella isabelina and its PUFAs extraction is the regulator of adipocytokines, we analyzed the epididymal white adipose tissue (WAT) from dyslipidemic or type II diabetic mice. The semi-quantitative RT-PCR has shown that Mortierella isabelina and PUFAs treatment significantly up-regulated leptin mRNA levels, the pattern of adiponectin mRNA regulation by fungi powder and PUFAs extraction was similar to that seen for leptin, and moderately down-regulated resistin transcript levels, though this change was...