Experimental Study On The Spleen-asthenia Syndrome Animal Model By Multiple-factor Over-fatigue Method

Since Beijing Normal University established rhubarb laxation spleen-asthenia Syndrome (SAS) animal model in 1980, more than ten animal models have been established. With the establishment of these animal models, it is possible to launch the further research of SAS. Now researchers have discussed symptoms of SAS animals in digestion and absorption, immunity, nerve and endocrine etc. However, SAS animal models in lab are much different from clinical SAS. According to traditional Chinese medical theory, as cause of illness, over-fatigue includes overwork, restlessness, and over- coitus. But current fatigue SAS model makes animals fatigued by over-exercise. And it has little influence on spirit of animals. Fatigue model only simulates overwork, but not restlessness. Therefore, it is quite different from clinical SAS. On the other hand, most SAS models only consider single factor. Only a few models include several factors. Accordingly, it is greatly necessary to improve current fatigue SAS model with single factor, and establish a new SAS model that similar to clinical SAS to study the essence of SAS.In order to simulate illness causes including overwork and restlessness, we make animals over-swimming, as is done in normal SAS models, and partially deprive animals of their rest time at the same time. Animals are not only tired but also restless. Based on this, we established an improved SAS animal model, namely improved over-fatigue mice SAS model.The change of general physiological characters, D-xylose excretion, activity of amylase in serum, stomach emptying and enterokinesia speed, contractile activity of small intestine in vitro, morphological change of gastrointestinal mucosa, gastrin (Gas) content in serum, spleen index, thymus index were observed. In order to evaluate our improved over-fatigue model, we compare it with rhubarb laxation SAS model and over-swimming SAS model, and proved by Sijunzi decoction. Moreover, we elementarily studied the mechanism of the changes in digestion and absorption function by improve over-fatigue method.The results showed improved over-fatigue mice represented typical SAS.Moreover, changes in objective indexes are consistent with literature. These changes have a coherent trend to those of SAS clinical patents, and most changes were more remarkable than the rhubarb root laxation and over-swimming SAS mice models. But only partial changes of rhubarb laxation group and over-swimming group have a coherent trend to those of SAS clinical patents. Our improved over-fatigue method is better than rhubarb laxation method and over-swimming method. Proved by Sijunzi decoction treatment, the mice behave normally. All objective indexes are improved more distinctively than the group that resumes naturally. These prove that the SAS model is made successfully and meets the requirements of judgement for SAS animal model. In this model, SAS is caused possibly by largely released Gas, which results in increased contractile in small intestine, over-exudation of intestine and obstacle in absorption. These changes are also basic pathologies of SAS symptoms.As a new SAS animal model, it simulates several SAS causes, approaches the pathologic changes of clinical patients. It is a more appropriate model to study the essence of SAS.