SA Cationic Liposomes Complexed To Plasmids Encoding Endostatin And P53 Inhibit Lewis Lung Cancer In Mice Model

Objective: To evaluate the inhibitory effection of SA cationic liposomes complexed plasmids encoding endostatin and/or p53 on Lewis lung cancer in mice model. Methods: Establish Lewis lung cancer mice model, administrate intratumorally SA cationic liposome complexed plamids encoding endostatin and p53, twice a week, for 6 weeks in all. Evaluate the function of inhibiting tumor and antiangiogenesis according to the size change of the tumor, the activity,food taking,nourishment,the existent time of the mice and metastasis in lung。Result:1,Lumps in the control groups grow continually ,while lump in the treated groups grow slowly after the third day. On the 18th day after treating , the tumor size of p53,pEnd,pEnd + p53 are 77±10mm3,39±10mm3,48±10mm3 respectively, while the tumor size of blank and SA-liposome control group are 4685±176mm3 and 6054±213mm3 respectively. the result shows magnificent statistical significance. (P<0.01).2,The activity, nourishment of the mice in all treatment group have no obvious changes, however the mice of two control groups become bad gradually. 3,metastasis in lung of p53,pEnd,pEnd + p53 group are 3.5±1.2,0±0.0,0±0.0 respectively,the blank group and SA-liposome control group are 18.4±5.1 and 17.1±4.7, the result shows statistical significance. (P<0.05). 4,The average existent period of all treatment group(above 50 days) is longer than blank control group(28.2±4.1) and SA-liposome control group(31.2±3.7) the result shows statistical significance. (P<0.05). Conclution : Administrate intratumorally cationic liposome complexed plamids encoding endostatin and p53 can powerfully inhibit the growth and metastasis of implanted Lewis lung cancer in mice model.