Effect Of Human Recombinant PLD2 In Vivo On Serumal GPI-PLD As Well As The Expression Of Nuclear Transcription Factor P65 In Chronic Asthma Of Guinea Pigs

Objective: In order to investigate the effect of human recombinantphospholipase D2 (rhPLD2) in vivo on the secretion of serumal GPI-PLD as well as the expression of nuclear transcription factor P65 in chronic asthma of guinea pig.Methods: After treatment the guinea pigs attacked by chronic asthma withrhPLD2, the GPI-PLD activity detection was carried out by phase separation of human placental alkaline phosphatase in Triton X-114. And the crude nuclear extraction was detected with TransAM Transcription Factor Assay Kits for pulmo tissue nuclear transcription factor P65.Results: Compared with the healthy guinea pigs, the serumal GPI-PLD andthe expression of nuclear transcription factor P65 in the guinea pigs in chronic asthma are much higher than control groups. Our results show that rhPLD2 can significantly reduce the secretion of GPI-PLD, and markedly depress the expression of P65 when the guinea pigs were attacked by chronic asthma.Conclusions: The human recombinant phospholipase D2 may suppress the chronic inflammatory reaction through reducing the release of serumal GPI-PLD and the expression of P65.