| Objective: To consummate model of rats induced by severe acutepancreatitis. Methods: A modified traditional intrapancreatobiliary duct injection method was adopted using I.V.Catheter and microsyringe to make SAP models. To avoid intestinal juice leak into belly cavity from duodenum by sewing the puncturing site of duodenum with absorption line in operation, and decreased the loss of fluid induced by not pancreatitis factor. The operated rats were injected physiological saline in the post period, in order to reduce mortality. The level of serum amylase, TNF- α and DL-6, and pathological changes of pancreas, are determined to evaluate the existence of pancreatitis. Results: In our experiment we observed that the level of serum amylase, TNF- α and IL-6, and the grade of pathological changes of pancreas of model group increased than sham operation group, and all rats of model group had the special pathological changes of pancreas for SAP 12 hours after operation. Conclusions: In our experiment we observed that Using I.V.Catheter could simplify the procedure and reduce the difficulty. We can inject the drug with the constant pressure and velocity by using microsyringe. To avoid intestinal juice leak into belly cavity from duodenum by sewing the puncturing site of duodenum with absorption line in operation, and decreased the loss of fluid induced by not pancreatitis factor, it increased the dependability and stability of the model group The NS we gave after operation reduced mortality. The model induced by SAP that we established successfully provides a method for further research of SAP. |
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