Relationship Between Expression Of Apoptotic Gene And Cellular Apoptosis In Placenta Of Fetal Growth Restriction

FGR(fetal growth restriction)is one of the leading causes of perinatal morbidity and mortality and also one of the main complications during the perinatal period. FGR not only affects fetal normal development, but also plays a role in the physical and mental development in the childhood and puberty. With the progress of the society, people's awareness towands eugenics have universally ascended to a new level, and they generally attach great importance to the perinatal mortality and the factors that affect fetal development. Unfortunately, in the last century ,the etiology and pathogenesis of FGR remain an enigma. It brought lots of troubles for the prevention and treatment of FGR. In the recent years the researchers found that FGR was a common complication of PIH (pre-gnancy-induced hypertention, now it was called hyper-tension disorder complication pregnancy),anemia, olig-ohy-dramnios and candical diseases. Among those condi-tions, PIH is the most common and the leading factor, so in the experimentation part of the samples we chose are PIH coupled with FGR. In the terms of pathogenesis of FGR, the placeatal cellular apoptosis and relevant genetic regulation are gaining increasing attention from lots of researchers. The cellular apoptosis and necrosis are two different concepts. Apoptosis is a gene-regul-ated-programmed cell death. It acts as a basic physical event, taking place in all kinds of physical and pathologic conditions. In the research of placental apoptosis gene, researchers have confined that apoptosis is present in placentas from the patients with FGR. This gene controls cellular apoptosis, therefore it leads to the pathologic changes of embryo. Nevertheless the pathogenesis of FGR is far more than complicated. It is probable a multi-gene-controlled disease, which is also subject to environ-mental factors. At present, the prospective genes that are relevant to FGR have been identified, and the total number is 10. However, which gene is present in the placental tissues, how about their gene expression and how the genes contribute to the apoptosis, for above questions we lack systematic research both at home and abroad. This experimentation intends to observe the apoptosis of the patient with FGR using light microscope and transmission electron respectively. We explore the degree of gene expression of P53, bcl-2 and bax and how they faciliate the apoptosis to explain the pathogenesis of FGR. For the sake of gaining some theorical support for the predictive prevention, clinical diagnosis and treatment of FGR. As for the FGR and HDCP, their pathogenetic, path-ophysiology and the histological and monphological change of embryos are interacted and integrate, the research of apoptosis in the patients which FGR can shed lights on that of HDCP. Materials and methods I collected the placental tissues of 36 patients withPIH complicated FGR and 38 normal full term labor women and made a comparison between the two groups of the length of gestation, gestational age, times of delivery, heights and ways of delivery. These are no statistical signify-cance. First I observed the weights of the placentas and the neonatal babies. Of the 36 patients with FGR and the 38 normal full term labor women, then made a measurement of the apoptosis in the placental tissues using light and transmission electron microscope to make sure if the apoptosis is present in the above samples, then I adopt immunochemistry and histochemistry techniques to explore the gene expression of P53, bcl-2 and bax. Consequences/Results ①In the FGR group, the mean neonatal baby weight is (2 062±432)gram, and the mean placental weight is (230±22)gram. In the comparative group , the new-born baby weight is (3 318±592)gram and the mean placental weight is (360±65)gram(P<0.05).There is statistical signify-cance in the two group.②The apoptosis is present in norm-al cells, the trophoblastic cells and the stromal cells of patients with FGR especially in the syncytical tropho-blastic cells. ③The experimentation prones that the expression levels of bcl-2 protein in both the placental tissues of the patients with FGR and the comparative group, there is statistical significance ( P=0.006 < 0.05). The...