Antimicrobial Activity And Toxicity Test And Clinical Observation Of The Drug Membrane For Oral Lichen Planus

Objective :To study the antimicrobial activity and toxicity of the drug membrane for OLP and to observe clinical effect in the treatment of OLP.Methods: Antimicrobial activity were determined. Kunmin mice were perfused stomach with 0.8mL suspension for acute toxicity test. Local stimulation test : the different pellicles were put on the underlip mucosa for 4 hours for observing the change of underlip mucosa. After 24 hours and 7 days, underlip mucosa and esophagus mucosa of rabbits were cut for pathology. Clinical observation :82 cases diagnosed as OLP were divided into two groups at random. The test group were administered by the drug membrane for OLP and the control by the compound chlorhexidine dexamethasone pellicles for one month. The following symptom and signs: diameter of lesion, pain, et al were evaluated 1,2,4 weeks after treatment. Statistic analysis was performed by SPSS.Result: The minimum inhibitory concentration of Salvia miltiorrhiza Bge to Staphylococcus aureus ATCC 25923, Streptococci beta-haem ATCC 32210, Porphyromonas gingivalis ATCC 33277 is 15.62mg/mL,15.62mg/mL,31.25 mg/mL and that of CM-chitosan to Staphylococcus aureus ATCC 25923 is 25 mg/mL. Salvia miltiorrhiza Bge does not produce antimicrobial ring to Candida albicans ATCC 76615 as CM-chitosan to Streptococci beta-haem ATCC 32210, Porphyromonas gingivalis ATCC 33277, Candida albicans ATCC 76615. The drug for oral lichen planus did not influence on the growth of mice(MTD>l 62.01 g/kg). The drug membrane did not cause erythema, oedema, erosion or uler to the underlip mucosa and esophagus mucosa of rabbits. And there is not abnormal in the pathology. The efficiency of the treatment and the control is 80.49% 41.46% respectively. Statistically significant difference was found in the clinical research 1,2,4 week after treatment (P<0.05). The result showed the drug membrane for OLP had better efficient to decreased diameter of erosion and white stripe than to that of the control group.Conclusion: The membrane is safety and efficient as a topical applied drug.