Effect Of Tetrahydrobiopterin And Folic Acid On Lipid Peroxidation. In Hypercholesterol Rabbits

Objective: We compaired the effects of tetrahydrobiopterin with folic acid on lipid peroxidation in Hypercholesteril rabbitsMethod: 24 rabbits were randomly devided into 4 groups: normal diet (group A); hypercholesterol diet (group B); tetrahydrobiopterin plus hypercholesterol diet (group C); folic acid plus hypercholesterol diet (group D). after 60 days, serum total cholesterol, superoxide dismutase and maleic dialdehyde were determined.as well as urinalysis.detect concentration of urinary pterin. Data was disposed by statistical software SPSS 11.0. P<0. 05 means statistical significance. P<0. 01 means observably statistical significance.,Results: The amount of total cholesterol in group B is the highest among these groups (p<0.01). Result proved the model of Hypercholesteril rabbits was established successfully. The activity of superoxide dismutase in groupB is lower than that in group A.C and D (p<0.01). The amount of maleic dialdehyde in group B is the highest(p<0.01) . there is no statistical difference of superoxide dismutase and maleic dialdehyde between group C and group D. Compaired with other three groups ,biopterin is obviously enhanced, whereas neopterin is lower in group B(p<0.05).Conclusion: It suggests that tetrahydrobiopterin and folic acid can reduce all kinds of oxygen free radicals and decline the level of lipid peroxidation. There is no obvious difference between them. Both of them can increase concentrstion of tetrahydrobiopterin in hypercholesterol rabbits (through detect Urinary pterin).Sequentially pterin).Sequentially play a role of antagonizing lipid peroxidation and preserving endothelial function It suggest that folic acid substitute tetrahydrobiopterin to treat cardiovascular disorder is feasible.