Alteration And Roles Of Soluable Vascular Endothelial Cell Growth Factor Receptor 1 In Preeclampsia

Preeclampsia is one of the common complications of pregnancy, resulting in substantial maternal and neonatal morbidity and mortality. Symptoms of preeclampsia were thought to be due to endothelial cell dysfunction. However, the cause of endothelial cell dysfunction remains unknown.We have recently demonstrated that gene of soluable vascular endothelial cell growth factor receptor 1 (sVEGFR-1) was up-regulated in preeclamptic patient placenta using suppression subtractive hybridization (SSH). However, further study is necessary to confirm the alteration of sVEGFR-1 in preeclampsia and understand the its roles in this disease. ObjectivesTo investigate the expression of sVEGFR-1 in placenta and level of serum sVEGFR-1 in preeclampsia patients and discuss the its roles in pathegenesis of preeclampsia MethodsSemi-quantitative RT-PCR was carried out to detect the level of sVEGFR-1 mRNA in placental of 10 preeclampsia (study group) and 10normotensive pregnancies (control group). ELISA was used to detect the serum level of sVEGFR-1 in peripheral venous blood and uterine vein blood in control group (n=35) and study group (n=35).The serum level of sVEGFR-1 in in early, middle and later pregnancies was measured, as well. The location of sVEGFR-1 mRNA in placenta was detected by in situ hybridization ResultssVEGFR-1 mRNA level of placental was significantly higher in preeclampsia than that in normal pregnancy. The serum level of sVEGFR-1 of peripheral vein in preeclampsia was significantly elevated, campared with that in normal pregnancy (5639.6 ± 3190. 5pg/ml VS2194. 4 + 635. 8 pg/m1, P<0. 01), so did the serum sVEGFR-1 of uterine vein in preeclampsia and control group (7673.3 + 2295.8 pg/ml vs 3056. 6 + 784. 6 pg/m1, P<0. 01). The sVEGFR-1 level of uterine vein blood was significantly higher in either preeclampsia or normal pregnancy than that of peripheral venous blood (P<0. 01). The serum level of sVEGFR-1 in early, middle and later pregnancy were 31.7 ±19. 6pg/ml(n=10), 994. 0 + 302.1 pg/ml (n=10), 2194. 4±635. 8 pg/ml (n=35), respectively, suggesting that the level of sVEGFR-1 in peripheral venous blood increased with the development of pregnancy (P<0.01). sVEGFR-1 mRNA was located in trophoblast of placenta by in situ hybridization. ConclusionsPreeclampsia has higher level of serum sVEGFR-1 than normal pregnancy. The upregulation of sVEGFR-1 expression in placenta and...