The Study Of The Expression Of MRP1 Gene In Refractory Epilepsy

Objective: To investigate the expression of MRPl gene in the peripheral blood of refractory epilepsy patients and discuss its significance.Materials and methods: The patients were divided into 4 groups: refractory epilepsy (Group A), epilepsy under control by anti-epileptic drugs (AEDs) (Group B), epilepsy without AEDs (Group Q and health adults (Group D). The expression of MRPl in their peripheral blood cells was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: The expressions of MRPl in the four groups are all positive. By semi-quantitative RT -PCR, we detected that the mean relative expression of MRPl mRNA in the peripheral blood of refractory epilepsy patients (Group A) was 0.776±0.016, and that in Group B, C and D were respectively 0.627±0.009, 0.642±0.010 and 0.625+0.006. Analyzed by one-way ANOVA, it meant that the expression of MRPl mRNA in the peripheral blood cells of refractory epilepsy patients was much higher than that of the patients in other group, and the difference was of significance (P< 0.001).Otherwise, the differences between the other three groups were of no significance (P>0.05).Conclusion: MRPl gene may play an important role in the multiple drug resistance (MDR) of refractory epilepsy. However, only drug induction or seizure itself may not result in up-regulation of MRPl expression in the peripheral blood.