Expression And Correlation Analysis Of â–³Np63 And MDM2 Proteins In Transitional Cell Carcinoma Of Urinary Bladder

Objective To explore the expression and significance of â–³Np63 and MDM2 proteins in the transitional cell carcinoma (TCC) of urinary bladder, especially the relationship between them.Methods Immunohistochemical method was used to detect the â–³Np63 and MDM2 proteins expression in 35 cases with the TCC of urinary bladder. 38 cases with normal transitional cell epithelium of urinary bladder were used as comparison.Results The positive rate of â–³Np63 and MDM2 protein expression in the TCC of urinary bladder were 83.3% and 42.9% respectively; In the normal transitional cell epithelium, the positive rate of â–³Np63 and MDM2 protein expression were 18.4% and 7.9% respectively. The positive rate of â–³Np63 and MDM2 protein expression in the TCC of urinary bladder was higher than the rate in normal transitional cell epithelium (p<0.01 ) . The positive expression of A Np63 protein in poorly differentiated and advanced TCC was significantly higher than well differentiated and superficial TCC (p<0.01); The positive rate of MDM2 protein expression in well differentiated and superficial tumor was significantly higher than poorly differentiated and advanced tumor (P <0.01). There was significant negative correlation between the expression of â–³Np63 and MDM2 protein(P=0.021).Conclusion The positive rate of â–³Np63 and MDM2 protein expression in the TCC of urinary bladder was higher than the rate in normal transitional cell epithelium, and the difference of the positive rate of â–³Np63 and MDM2 protein expression between every stages and classes has significant statistical meaning simultaneously ,which suggested that â–³Np63 and MDM2 are both involved in the occurrence and progress of TCC. â–³Np63 and MDM2 are an early and a late gene events of TCC, so they can be used to diagnose TCC earlier and to judge the degree of malignancy. Combination of these two genes can provide more biological information of TCC.