N-[4-hydroxyphenyl] Retinamide (4-HPR) Increasing The Radiotherapy Sensitivity On Bladder Cancer Cell Line

Objective: To study the growth suppression and apoptosis induction combined effect of 4-HPR and γ -radiation on bladder transition cancer cells and to analysis whether there would be synergistic action. Methods: MTT colorimetric assay was applied to examine the growth status of the cells. Flow cytometer was used to detect the growth inhibition effect of 4-HPR and/or γ-radiation on bladder cancer cell line T24. Transission electron microcopy was employed to observe morphological transformation of the cells.ResuIts: The apoptosis ratio of T24 cells on day 5 with 2.5μmol/L, 5μmol/L and 10μmol/L 4-HPR-treated was 12.17%,28.0% and 26.76%, respectively, and the mortality rate were 3.26%,12.35% and 26.66%,respectively. In the group of treatment with 2.5μmol/l or 5μmol/l 4-HPR combined lOOcgy γ -radiation, the apoptosis ratio on day 3 was 20.00% and 26.00%, and the mortality rate was blow 5.0%, whereas the apoptosis ratio were 38.00%, 35.00%, and the mortality rate were 12.35% and 21.13% on day 5. The junction...