Establishment Of Human Hepatocarcinoma Multi-drug Resistant Strain And Stable Expression Of MDR1

Objective To establish Multi-drug resistant strain stably expressing human hepotacarcinoma Bel-7402 and to study its multi-drug resistance mechanism to provide laboratory models for application of RNA interfere technique in the fight against drug resistance tumors.Methods Bel-7402 Multi-drug resistant strain was established by long - term culturing and screening of adriamycin. And the following techniques, namely, MTT, FCM and RT-PCR were used systemically the relevant features of the strain, including extent of drug tolerance, lethal dose, function and positive rate of pgp and test of gene MDR1.Results MTT showed that the Multi- drug resistance of strain increased significantly FCM found, From sensetive cell to Multi-drug resistance cell, with the function of PgP falling from 77.7% to 24% and positive rate of pgp rising from 1.35% to 54%. PT-PCR revealed that Multi-drug resistance cell strain had MDRmRNA over expression.Conclusion Multi-drug resistant strain that stably express human hepatocacinoma Bel-7402 cell was successfully established. The strain has high expression of pgp and high stability.