The Effect Of Recombinant Human Growth Hormone On Bone Marrow Hematopoietic Function In Intraperitoneal Chemotherapied Rats

Objective To investigate the effect of recombinant human growth hormone (rhGH) on bone marrow hematopoietic function in chemotherapied rats. Methods Analysis of peripheral blood by automatic blood cell counter, smearing for bone marrow cells, pathological section for bone marrow and the expression of proliferating cell nuclear antigen (PCNA) in bone marrow by immunohistochemical method Results Day 7 after administration, reticulocyte count had obviously increased in rhGH group (P<0.05), while the white blood cell (WBC) count, hemoglobin and reticulocyte count was decreased distinctly (P<0.05) in chemotherapy group and chemotherapy+ rhGH group, however, the decreased degree in chemotherapy+ rhGH group was less than that in chemotherapy group (PO.05). in myeloid morphology, myeloid cell was hypoplastic excessively hi chemotherapy group, and it was hypoplastic obviously in chemotherapy+ rhGH group. Myeloid karyote count was dropped significantly and the proportion of non-hcmatopoietic cell was raised distinctly both in chemotherapy and chemotherapy+ rhGH group (PO.05). But there were no myeloid granules and few megakaryocyte in chemotherapy group, while some myeloid granules, megakaryocyte and polychromatic erythrocyte could find in chemotherapy+ rhGH group. Day 14, the counts of red blood cell (RBC) and hemoglobin were dropped distinctly in chemotherapy group and chemotherapy+ rhGH group (P<0.05), but the decreased degree in chemotherapy group was more thanthat in chemotherapy rhGH group (P<0.05). Reticulocyte count of chemotherapy+ rhGH group was higher than others (P.005). There was no statistical significance in WBC count (P >0.05). In myeloid morphology, myeloid cell was hyperplastic actively excessively in chemotherapy and chemotherapy rhGH group, and myeloid karyote count was increased significantly in chemotherapy+ rhGH group than that in chemotherapy group (P<0.05), and morphology of myeloid series was recovered. Day 21, RBC and hemoglobin count hi chemotherapy group was less than control group and rhGH group (PO.05), and hi chemotherapy rhGH group the counts of RBC was lower than rhGH group (P<0.05), while reticulocyte count was higher than others still (PO.05). Day?, 14 and 21, PCNA positive cells count hi chemotherapy group was lower than rhGH group and chemotherapy rhGH group (PO.05). Conclusion rhGH has a protective effect on myeloid hematopoietic function hi intraperitoneal chemotherapied rats.