Study On The Procedure And Factors Of Granulocytes In Bone Marrow Releasing To Peripheral Blood After Chemotherapy

Objectives:To study the barrier in process of granulocyte in bone marrow of mice releasing to peripheral blood after chemotheraphy, and seek the best intervention with different agents to promote the granulocyte release.Methods:After intraperitoneal injection of cyclophosphamide(CTX), mice were treated with granulocyte-colony stimulating factor (G-CSF), traditional Chinese drug of astragalus polysaccharide (APS), dexamethasone(DXM) and anti-L-selectin monoclonal antibody (McAb) respectively. Proliferation status of bone marrow was assayed by Wright's stained bone marrow smears of sternum and negative rate of L-selectin on granulocyte in bone marrow was assayed by immunohistochemistry of APAAP;serum sCD62L concentration was measured by ELISA and peripheral blood white blood cells were counted at different time of chemotherapy. The Comparison and correlation of three factors were analyzed by statistical methods.Recult:1. Peripheral blood white blood cell count reduces when proliferation of bone marrow is active on 7, 10 and 14 days of chemotherapy, is 2.02±0.27×10~9/L, 2.96±0.65×10~9/L, 3.43±1.37×10~9/L respectively ( 6.13±0.70×10~9/L befor chemotherapy), as well as negative rate of L-selectin on granulocyte in bone marrow has no change on 7 days (P>0. 05) and elevates on 10 and 14 days (P<0. 05) . And the two factors had positive correlation, r=0.8988, P<0.05.2. G-CSF, APS, DXM and McAb can increase the peripheral blood white blood cell count after chemotherapy, and APS is the best in prevention with the lowest level of3.48±0.25xl09/L which is double than that of chemotherapy group after 24 hours of chemotherapy(d4). The peripheral blood white blood cell count of APS in prevention remains the level from d7 to dl4. At the same time, he negative rate of L-selectin on granulocyte in bone marrow also correspondingly increases in treatment groups.3. The extent of serum sCD62L concentration is 1. 02~2. 08 u g/ral and increases gradually during ganulocyte in bone marrow releasing to peripheral blood in APS and G-CSF groups,but decreases during immediately releasing of DXM and McAb.4. Peripheral blood white blood cell count and negative rate of L-selectin on granulocyte in bone marrow present the positive correlation in chemotherapy , G-CSF and APS group.5. Peripheral blood white blood cell count and serum sCD62L concentration is positive correlation in G-CSF group and also equidirectional in other groups on 7, 10 and 14 days of chemotherapy, but negative correlation in blank and McAb groups.Conclusions:1. There is the barrier of granulocyte in bone marrow releasing to peripheral blood on 7 days of chemotherapy.2. G-CSF,APS,DXM and McAb can promote granulocyte to release after chemotherapy, and APS is the best in prevention.3. Peripheral blood white blood cell count and negative rate of L-selectin on granulocyte in bone marrow present the positive correlation when granulocyte in bone marrow releasing to peripheral blood is inhibited after chemotherapy.4. Serum sCD62L concentration maintains at some stable extent in vivo to against transient and intensive fluctuation by itself regulatory mechanism during ganulocyte in bone marrow releasing to peripheral blood.But the mechanism is weak when fluctuation of serum sCD62L concentration is moderately.