| Human genome is composed of 22 autosomes and 2 sex chromosomes and its encodeing genes takes the whole inherited information correlating with growth, development, decrepitude and death of human anybody. Human genome is a very resistant architecture which ensures the coincidence and stability of human as a species. Human genome is also a variant system and the variations of DNA sequence take place unceasingly during the long-term evolution. Some of those variations have been reserved which give rise to the differences and polymorphisms among different races and individuals.The performance of HGP(human genome project)makes it possible to comprehend the variations and polymorphisms of genes among different races and different individuals. The study on polymorphism marker can be helpful to reveal not only the association between variation and genotype but also the association between variation and risk of disease. It is very important to the preventions, diagnoses and treatments of diseases, especially the complicated ones. Pregnancy-induced hypertension syndrome (PIH) is a specific disease of the gestation and it is the leading cause of perinatal morbidity and mortality. However the exact patho- physiological process of PIH still remains to be unrevealed so it is very important to study the etiology in order to prevent and cure PIH. Now there are many research on renin-angiotensin system(RAS) of human AT1R gene. Pathocological changes of PIH are all of small arteries spasm and damage of endotheliocytes. Main expressions are the rising of blood pressure, proteinuria and edema ,resulted from mechanism disorder.Reseach show RAS play an important role in mainting water-salt balance and modulating heart vascular disease, involved in the process disease. The main process: angiotensinogen is the perform of angiotensin and transformed into angiotensin I by the rennin. Angiotensin I is changed into biological action. Angiotensin II, which has the strong function of constraction blood vessel.However, angiotensin II has to combine with ATR, especially with AT1R to perform its functions. The change of molecular biological of this receptor main cause the rise of blood pressure. PIH has a strong heredity tendency and gene mutation may be the most important pathogenesis. Investigations have shown that several different kinds of polymorphisms in human AT1R gene have been detected. Despite numerous studies shows no genetic variant has been identified in AT1R,which shows a strong genetic role in Asian essential hypertensive populations. Takahashi, et al made the research on AT1R 5'-UTR(7 polymorphism among the hypertension samples) and found that a significantly higher frequency of the AT1R(-535) T allele was observed in hypertensive subjects. Evidence was obtained that AT1R(-535) T allele showed a synergistic effect on risk of hypertension with angiotensin I converting enzyme D allele (ACE).Because the main process of PIH is hypertension and body mechanism disorder. It has the similar pathological changes with hypertension and provide the research basis between this polymorphism and PIH by study 7 polymorphism with hypertension. Therefore the investigation of AT1R genetic polymorphism will help to clarify molecular genetics mechanism of PIH.Methods: In our study, 86 patients with PIH and 74 normal controls, matched for age and week of gestation, were recruited. DNA was extracted from peripheral blood. Genotyping was performed by using PCR-based restriction fragment length polymorphism(RFLP) method. The products of enzyme digestion were separated by electrophoresis in 2.5% agarose gel, dyed with EB and analysed by gel aerial image.All data were analysed with spss software.Results:(1)There is no statistically significant difference between the frequencies of AT1R CC,CT and TT genotypes, 70.93%, 23.26% and 5.81% respectively in PIH group compared with 87.84%,9.46% and 2.70% respectively in normal control group(P>0.05).There is significant difference between the frequencies of the two al... |
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