Generation And Characteristic Study On Cytokine-induced Killer Cells From Peripheral Blood Mononuclear Cells Of Patients With Acute Myeloid Leukeamia

Cytokine-induced killer(CIK) cells can mediate highly efficient non-major histocompatibility complex(MHC)-restricted killing of tumor cell targets and proliferate rapidly in vitro. In contrast to the LAK cells that are activated CD3"CD56+ NK cells and the TIL that are essentially CDS cytotoxic T cells, CIK cells are distinct and express both the CD3 and CDS 6 cell surface markers. The high lytic activity of CIK cells correlates with a high expansion of CD3 and CD56 double positive cells. Linn reported the expansion of CIK cells from the bone marrow samples of patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).But it is difficult to gain BM sample enough to induce the CIK cells. Here we explore the possibility of generation of CIK cells from the peripheral blood mononuclear(PBMC) cells of patients with AML.First we compared the effects of exogenous IL-1. IL-2 or IL-12 used for generation of CIK cells on proliferation . cytotoxicity and antigen presentation. Then a optimal protocal was selected to apply for the generation of CIK cells from PBMC of patientswith AML. Last we examined the cytokine profiles of CIK cells to explore the possible functional mechanism of CIK cells. In conclusion, three methods mentioned can be used to generate immuno-effector cells for immunotherapy. Expansion of CIK cells resulted from AML may becomes a new immunology therapy for AML. CIK cells may regulate the immune response by Thl cytokine profile.