Experiment Study About Selenium Nutrition Treatment On Diabetes Mice

Objective:To investigate the effect and possible mechanism of selenium cure metabolize turbulence on diabetes mice, choose feasible estimate index of curative effect, and discuss the excellent or inferior of supply selenium forms primarily.Methods:Use 19.09±1.3 g ,male Kuming mice were feed three days to adapting circumstance ,and were randomly taken out twenty four mice into two groups,after 24 hours fasting ,the odd mice were injected 180mg/kg weight ,2% alloxan in abdomen to make diabetes model(DM),and the two groups mice were injected correspond cubage 0.9% brine in abdomen. After injection ,resume to serve food and water,and measure three urine glucose at 5,10,14 day.According to urine glucose level, It's continue to present three " + " that can be regarded as become diabetes model (DM) ,then divided randomly diabetes mice into four groups,each group have seventeen mice.Experiment have six groups: normal control group (Ⅰ),normal+ selenoprotein group(100μg Se /kg weight) (Ⅱ), DM control group (Ⅲ),DM+ lower dose selenoprotein group(100μg Se /kg weight) (Ⅳ), DM+ higher dose selenoprotein group(300μg Se /kg weight) (Ⅴ),DM+ Na2SeO3 group(100μg Se /kg weight) (Ⅵ). Selenium was supplied except selenium in ordinary feedstuff,and the selenium level of ordinary feedstuff is 0.159 mg /kg by our lab mensurating. The way of feeding selenium is by drinking liquid.We measure once weight and thrice dose of take food and drink water of mice every week,and adjust once thickness of supply selenium liquid by measure average drink water dose and weight of every group every week. Mice free eating and drinking, the supplied feedstuff is ordinary feedstuff. The normal group drink tap water , Ⅱ～Ⅵ groups drink supply selenium liquid by tap water dissolving. Selenoprotein liquid were confect everyday and Na2SeO3 liquid were confect every week. After supplied selenium eight weeks,mice were forbided eating to 12 hours,were taken blood by extirpating eyeball, after resisted cruor , were separating blood plasma from blood in time to be keep in low temperature.Then ,break neck to kill mice,and extirpate part of liver ,put into 10% formaldehyde liquid for fixation and used for pathology examination by HE and PAS dyeing. The odd liver and kidney wash by cool 0.9% brine and sip up by filter paper,then to keep in low temperature,used to measure these index : Selenium content of liver, Hepatin content of liver and muscle, Na+-K+-ATPase and Ca2+-ATPase activity of kidney, GPX activity in blood ,and Glucose,MDA,NOS,NO,TG,TC of plasma, et al.Results:1. Compare with Ⅰ group(8.33±1.35 mmol/L), the blood glucose of Ⅱ group(5.93±1.64 mmol/L ) could be significantly decreased , p＜0.01 ; compare with Ⅲ group(45.29±3.26 mmol/L), the blood glucose of Ⅴ group(20.40±6.32 mmol/L ) could be significantly decreased , p＜0.01 ,but can't be dropped to the level of Ⅰ group; blood glucose among Ⅳ,Ⅴ and Ⅵ groups have not significant difference, P＞0.05. The selenium content of liver of Ⅱ group are higher thanⅠ group, P<0.05; the selenium content of liver of Ⅴ(1.40±0.26μg/g) and Ⅵ groups(1.52±0.35μg/g) are higher than Ⅲ group(0.99±0.31μg/g), P<0.05.2. Hepatin content of liver of Ⅲ group(6.78±2.22 mg/g liver) are higher than Ⅰ group(2.14±0.95 mg/g liver), P<0.01; Ⅳ,Ⅴ and Ⅵ groups compare with Ⅲ group , hepatin content of liver could be significantly decreased , P<0.01, and that can be dropped to the level of Ⅰ group, P＞0.05. Ⅲ group compare with Ⅰ group ,hepatin content of muscle have increase trend, but have not statistic significance, P＞0.05;Ⅳ,Ⅴ and Ⅵ groups compare with Ⅲ group, hepatin content of muscle have not statistic significance, P＞0.05.3. TC content in plasma of Ⅲ group(3.04±0.99 mmol/L) compare with Ⅰ group(2.00±0.26 mmol/L) have significantly increase, P<0.05;Ⅵ group(1.89±0.56 mmol/L) compare with Ⅲ group , TC content in plasma have significantly decrease, P<0.05,and that can be dropped to the level of Ⅰ group, P>.05. TG content...