| In Modern war and military training and performance, officers and soldiers usually have suffered from sports fatigue because long-time work or over loading training make their physical output and mental burden increased. sports fatigue can affect military training, performance and even military power. Anti-sports-fatigue is based on study on sports fatigue mechanism. So within the military medical domain, more and more researchers think highly of sports fatigue mechanism.Free Radicle is one of major factors in the sports fatigue. Free radicle which is produced during breather can attack other molecules in the body in order to grab an electron and damag the "healthy' molecule, which could cause cellular function abnormal. Therefore, we infer that addition of antioxidants can anti-fatigue. In fact, numerous animal experiments have demonstrated that the addition of antioxidants can improve locomotivity and postpone sports fatigue. Trace element Cu and Zn which belong to essential trace element of body play a very important role in antioxygen of body. Within sports medicine domain, the antioxidative potential of Zn and Cu was studied on restraint sports fatigue induced oxidant/pro-oxidant status. Everyday many athletes usually have supplementation with certain dosage of Zn and Cu in order to decrease sports fatigue and improve locomotivity. however, many studies have suggested that supplementation with Zn and Cu does not could decrease sports fatigue and improve locomotivity. Whether supplementation with Zn and Cu could decrease sports fatigue and improve locomotivity is still a matter of debate. So this is an important area for future research. Elucidation of the relationship of sports fatigue, free radicle metabolism and Zn,Cu nutrition is very significant for us to understand affect of trace element Zn and Cu to sports fatigue and decide nutrition requirement.In our research, the model of sports fatigued SD rats were established by ten days loading-increasing swimming exercise. Antioxidant capacity and the degree of oxidative damage of SD rat were observed.The Cu and Zn in liver,kidney skeletal ,muscle,serum of the SD rat were were analyzed by Flame atomic absorption spectrophotometry (AAS). speciation of the Cu and Zn in serum were analyzed by molecularbiology technology and AAS. At last, Relationship among trace Elements Cu and Zn , speciation of the Cu and Zn in serum and Metabolism of Free Radicl in SD Rat were analyzed.The main findings of the study are as follows:1.Under sports fatigued condition, the distribution of Cu and Zn in SD rat changed. The amount of Cu in the liver and serum of fatigued SD rat were increased compared with normal rats(P <0.05). The change of Zn in liver was also increased ,but the change of Zn in serum was opposite(P <0.05). The amount of Cu and Zn were decrease in the skeletal muscle(P <0.05) and did not change in the kidney compared with normal rats(P>0.05). 2.Under sports fatigued condition, antioxidant capacity of SD rat decreases. The activities of SOD in liver,kidney,skeletal muscle,serum were significantly lower and beside skeletal muscle the levels of MDA in tissue were significantly higher than control group(P <0.05).3.Under sports fatigued condition, the distribution of Cu and Zn in SD rat relates with antioxidant capacity. There are relationship between trace elements Cu,Zn and capability of antioxidation in the liver,skeletal muscle,serum of fatigued SD rat(P <0.05).4.A method was developed for speciation analysis of the Cu and Zn in serum. The detection limit of Cu in serum by the method is in the range 9.84×10-3μg·mL-1. For Zn, The detection limit is in the range 1.06×10-3μg·mL-1,The percentage recovery of trace elements Cu and Zn by the proposed procedure is in the range 95.0～103.0%. The relative standard deviation(RSD) of trace elements Cu and Zn in the serum is in the range 0.34%～2.31%.5. Under sports fatigued condition, the distribution of speciation of the Cu and Zn in serum of SD rat changed , which relates with capability of antioxidation of serum. The amount of forms of total, combination and non combination of Zn in the Serum of fatigued SD rat were increased compared with normal rats(P <0.05), but the changes of Cu were opposite. There are relationship among all kinds of forms of trace elements Cu, Zn and capability of antioxidation in the serum of sports fatigued SD rat(P <0.05).On the whole, sports could not only affect the metabolism of Zn and Cu, but also affect the metabolism of free radicle. In addition, the metabolism of Zn and Cu closely relate with the metabolism of free radicle. During exercise, the distribution of Cu and Zn in SD rat changed and the distribution of speciation of the Cu and Zn in serum of SD rat also changed. Those changes meet body's satisfaction of energy and material but cause decrease of capability of antioxidation. A lot of free radicles produced during exercise could cause sports fatigue. |
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