| IntroductionAngiogenesis is defined as the process of formatting a new capillary network . This capillary network comes from prior existential blood vessels by germination or ungermination style. Series studies prove that angiogenesis is important to tumor's growth and metastasis. The blood malignant tumor also have angiogenesis phenamena. The tumor's angiogenesis is relate to a series of growth factors, which stimulate endothelial cells to growth and excite the capillary network's generation.Vascular endothelial growth factor ( VEGF) is a specific mitogen to vascular endothelium. The VEGF family consists of VEGF - 1 , VEGF - 2 , VEGF - 3 , VEGF -4 and VEGFR - 1 ( fit - 1 ) .VEGFR -2 ( flk - 1/KDR ) .VEGFR -3 ( fit - 4 ). The recipients of VEGF family members are all tyrosine kinase recipients. Ras was an oncogene, which can make the normal cells convert to cancer ceU. In mammal animals, ras family has three members H - ras,K - ras, N - ras. The ras family members'code producers are all molecular weight 21, 000 protein, so call p21 protein. In blood system, N -ras is the most important. ERK ( extra cellular signal - regulated kinase ) has ERK1 , ERK2 two members.The study objects are 28 ANLL bone marrow specimens, 6 complete remission ANLL patients'bone marrow specimens, 15 CML bone marrow specimens, 6 other hematological diseases bone marrow specimens, 12 contrasts bone marrow specimens. We use immunohistochemistry method to detect VEGF, N -Ras,pERK protein level. The object is to detect VEGF,N - Ras,pERK protein level in leukemia and other malignancy hematologic disease and to discuss angiogenesis's adjustment mechanism. The final object is to made a background tofurther anti - angiogenesis study.Material and MethodsThe study objects are 28 ANLL bone marrow specimens, 6 complete remission ANLL patients'bone marrow specimens, 15 CML bone marrow specimens, 6 other hematological diseases bone marrow specimens, 12 contrasts bone marrow specimens. Bone marrow fluids is retreated with heparinization. Bone marrow is extracted mono - nucleus cell by Ficoll density gradient centrifugation. After the number cells with light microscope. The cells are smeared by cell smearing centrifugal machine. Specimens conservate in - 40t. The samples are incubated in room temperature 30 min. Detecting VEGF,N - Ras,pERK by immunohistochemistry method. By light microscope number positive cells, and calculate the positive ratio. We make statistics analysis by SPSS 11.0.Results. 1. The expression of VEGFDetection of VEGF by immunohistochemistry, preliminary diagnosis ANLL 鈥? CML VEGF expression rate are significantly higher than contrast bundle(P <0. 05). ALL ,complete remission ANLL and contrast bundle's VEGF expression rate have not significant difference ( P > 0. 05 ). Preliminary diagnosis ANLL VEGF expression rate is significantly higher than complete remission ANLL contrast bundle (P< 0.05).2. The expression of N - RasDetection of N - Ras by immunohistochemistry, preliminary diagnosis ANLL , CML N - Ras expression rate are significantly higher than contrast bundle ( P <0. 05). ALL,complete remission ANLL and contrast bundle's N - Ras expression rate have not significant difference ( P > 0. 05 ). Preliminary diagnosis ANLL N - Ras expression rate is significantly higher than complete remission ANLL contrast bundle(P <0.05 ) .3. The expression of pERKDetection of pERK by immunohistochemistry, preliminary diagnosis ANLL, CML pERK expression rate are significantly higher than contrast bundle(P <0. 05). ALL,complete remission ANLL and contrast bundle's pERK expression rate have not significant difference ( P > 0. 05 ). Preliminary diagnosis ANLL pERK expression rate is significantly higher than complete remission ANLL contrast bundle(P<0.05).4. The correlation of VEGF,N - Ras,pERK,VEGF,N - Ras,pERK all have correlation (P <0.01).DiscussionHuman leukemia is a typical disease by chromosomal mutation. N - ras mutation can lead hemopoietic progenitor cell to proliferation and los... |
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